Integration of transcriptomics reveals ferroptosis-related signatures and immune cell infiltration in bronchopulmonary dysplasia

Zhengyun Hu; Chong Liu; Yan Mao; Jianwei Shi; Jinwen Xu; Guoping Zhou; Feng Jiang

doi:10.1016/j.heliyon.2023.e21093

Integration of transcriptomics reveals ferroptosis-related signatures and immune cell infiltration in bronchopulmonary dysplasia

Heliyon. 2023 Oct 20;9(10):e21093. doi: 10.1016/j.heliyon.2023.e21093. eCollection 2023 Oct.

Authors

Zhengyun Hu¹, Chong Liu¹, Yan Mao², Jianwei Shi³, Jinwen Xu⁴, Guoping Zhou², Feng Jiang⁵

Affiliations

¹ Department of Pediatrics, Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine (Preparatory Stage), Shanghai, China.
² Department of Pediatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
³ Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.
⁴ Department of Pediatric Nephrology, Wuxi Children's Hospital, Wuxi, China.
⁵ Department of Neonatology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.

Abstract

Ferroptosis has emerged as a significant factor in the development of bronchopulmonary dysplasia (BPD). Nevertheless, our understanding of the potential involvement of ferroptosis-related genes (FRGs) in BPD remains incomplete. In this study, we leveraged the Gene Expression Omnibus (GEO) database to investigate this aspect. We identified 20 differentially expressed FRGs that are associated with BPD, shedding light on their potential role in the condition.LASSO along with SVM-RFE algorithms found that 12 genes: MEG3, ACSL1, DPP4, GALNT14, MAPK14, CD82, SMPD1, NR1D1, PARP3, ACVR1B, H19, and SLC7A11 were closely related to ferroptosis modulation and immunological response. These genes were used to create a nomogram with good predictive power and were found to be involved in BPD-linked pathways. In addition, the marker genes-based prediction model performed well in external validation data sets. The study also showed a significance between BPD and control samples in terms of immune cell infiltration. These findings may help improve our understanding of FRGs in BPD and lead to the development of more effective immunotherapies.

Keywords: Bronchopulmonary dysplasia; Diagnostic; Ferroptosis; Immune infiltration; Prediction model.