Comparative analysis of physiological traits and gene expression patterns in nitrogen deficiency among barley cultivars

Zohreh Hajibarat; Abbas Saidi; Habibollah Ghazvini; Zahra Hajibarat

doi:10.1186/s43141-023-00567-w

Comparative analysis of physiological traits and gene expression patterns in nitrogen deficiency among barley cultivars

J Genet Eng Biotechnol. 2023 Nov 10;21(1):110. doi: 10.1186/s43141-023-00567-w.

Authors

Zohreh Hajibarat¹, Abbas Saidi², Habibollah Ghazvini³, Zahra Hajibarat¹

Affiliations

¹ Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
² Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran. abbas.saidi@gmail.com.
³ Department of Cearal Research, Seed and Plant Improvement Institute (SPII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

Background: Nitrogen is one of the most important mineral nutrients for plants and is absorbed by the root system mainly in the inorganic form (NH⁺₄ and NO^-₃). Plants absorb nitrogen as a food source for growth, biomass production, and development. Nitrogen is mainly absorbed as nitrate, which is the most common source of nitrogen available to higher plants. One of the unique features of nitrate transport is that NO^-₃ is both a substrate for transport and an inducer of NO^-₃ transport systems in genes and at physiological levels.

Methods: In the present study, morphological and physiological traits (chlorophyll a/b, total chlorophyll, and carotenoid, antioxidant enzymes, and protein content), correlation between traits and gene expression, and principle component analysis of traits among five barley cultivars were measured in response to nitrogen deficiency (ND). The starved plants were transferred to a nutrient solution containing 0.2 mM and 2 mM NO^-₃ up to 7 and 14 days after ND application and non-stressed conditions, respectively.

Results: Gene expression analysis revealed that the 10 HvNRT2 genes were induced in the leaf and root tissues at 7 and 14 days after ND treatments in five barley cultivars. Expression of NRT2 genes by relative quantitative qRT-PCR analysis for 10 HvNRT2 genes were determined. Based on the gene expression, HvNRT2.1, HvNRT2.2, and HvNRT2.4 were strongly induced by NO^-3, peaking at 7 and 14 days after ND treatment. In contrast, the HvNRT2.4 showed only moderate induction in both leaves and roots. From our results, the Reyhan cultivar showed a significant increase in root fresh weight (RFW), protein content, and antioxidant enzyme activity in roots at 7 and 14 days after ND treatment as compared to the non-stressed condition. A highly positive correlation was observed between root catalase (CATr) and HvNRT2.2/2.5/2.6 leaves.

Conclusion: The expression of HvNRT2.4 is increased during long-term nitrogen starvation, while the expression of HvNRT2.1 and HvNRT2.2 are transiently increased by ND. Based on physiological and morphological traits and molecular mechanisms, the Reyhan is considered a tolerant cultivar under ND condition.

Keywords: Barley; Nitrate transporter genes; Nitrogen deficiency; Physiological traits.