Gut microbes modulate (p)ppGpp during a time-restricted feeding regimen

mBio. 2023 Dec 19;14(6):e0190723. doi: 10.1128/mbio.01907-23. Epub 2023 Nov 16.

Abstract

Mammals do not eat continuously, instead concentrating their feeding to a restricted portion of the day. This behavior presents the mammalian gut microbiota with a fluctuating environment with consequences for host-microbiome interaction, infection risk, immune response, drug metabolism, and other aspects of health. We demonstrate that in mice, gut microbes elevate levels of an intracellular signaling molecule, (p)ppGpp, during the fasting phase of a time-restricted feeding regimen. Disabling this response in a representative human gut commensal species significantly reduces colonization during this host-fasting phase. This response appears to be general across species and conserved across mammalian gut communities, highlighting a pathway that allows healthy gut microbiomes to maintain stability in an unstable environment.

Keywords: (p)ppGpp; Bacteroides; fasting; gut microbiota; stringent response.

MeSH terms

  • Animals
  • Bacteria / genetics
  • Bacteria / metabolism
  • Fasting
  • Gastrointestinal Microbiome* / physiology
  • Guanosine Pentaphosphate* / metabolism
  • Host Microbial Interactions
  • Humans
  • Mice
  • Mice, Inbred C57BL

Substances

  • Guanosine Pentaphosphate