The Emerging Role of Induced Pluripotent Stem Cells as Adoptive Cellular Immunotherapeutics

Biology (Basel). 2023 Nov 11;12(11):1419. doi: 10.3390/biology12111419.

Abstract

Adoptive cell therapy (ACT) has transformed the treatment landscape for cancer and infectious disease through the investigational use of chimeric antigen receptor T-cells (CAR-Ts), tumour-infiltrating lymphocytes (TILs) and viral-specific T-cells (VSTs). Whilst these represent breakthrough treatments, there are subsets of patients who fail to respond to autologous ACT products. This is frequently due to impaired patient T-cell function or "fitness" as a consequence of prior treatments and age, and can be exacerbated by complex manufacturing protocols. Further, the manufacture of autologous, patient-specific products is time-consuming, expensive and non-standardised. Induced pluripotent stem cells (iPSCs) as an allogeneic alternative to patient-specific products can potentially overcome the issues outlined above. iPSC technology provides an unlimited source of rejuvenated iPSC-derived T-cells (T-iPSCs) or natural killer (NK) cells (NK-iPSCs), and in the context of the growing field of allogeneic ACT, iPSCs have enormous potential as a platform for generating off-the-shelf, standardised, "fit" therapeutics for patients. In this review, we evaluate current and future applications of iPSC technology in the CAR-T/NK, TIL and VST space. We discuss current and next-generation iPSC manufacturing protocols, and report on current iPSC-based adoptive therapy clinical trials to elucidate the potential of this technology as the future of ACT.

Keywords: adoptive cell therapy (ACT); chimeric antigen receptor T-cells (CAR-Ts); induced pluripotent stem cells (iPSCs); manufacturing; natural killer (NK) cells; off-the-shelf; reprogramming; tumour-infiltrating lymphocytes (TILs); virus-specific T-cells (VSTs).

Publication types

  • Review

Grants and funding

This research received no external funding.