Distribution of Small RNAs Along Transposable Elements in Vitis vinifera During Somatic Embryogenesis

Methods Mol Biol. 2024:2732:279-286. doi: 10.1007/978-1-0716-3515-5_19.

Abstract

Metaviridae is a family of reverse-transcribing viruses, closely related to retroviruses; they exist within their host's DNA as transposable elements. Transposable element study requires the use of specialized tools, in part because of their repetitive nature. By combining data from transcript RNA-Seq, small RNA-Seq, and parallel analysis of RNA ends-Seq from grapevine somatic embryos, we set up a bioinformatics flowchart that could be able to assemble and identify transposable elements.

Keywords: Degradome; Small RNAs; Somatic embryos; Transposable elements; Vitis vinifera.

MeSH terms

  • DNA Transposable Elements* / genetics
  • RNA
  • Vitis* / genetics

Substances

  • DNA Transposable Elements
  • RNA