Delivery of external proteins into the cytoplasm using protein capsules modified with IgG on the surface, created from the amphiphilic two helix-bundle protein OLE-ZIP

Kousuke Takahashi; Taiki Nishiyama; Naoki Umezawa; Yasumichi Inoue; Isamu Akiba; Takehisa Dewa; Atsushi Ikeda; Toshihisa Mizuno

doi:10.1039/d3cc05347d

Delivery of external proteins into the cytoplasm using protein capsules modified with IgG on the surface, created from the amphiphilic two helix-bundle protein OLE-ZIP

Chem Commun (Camb). 2024 Jan 23;60(8):968-971. doi: 10.1039/d3cc05347d.

Authors

Kousuke Takahashi¹, Taiki Nishiyama¹, Naoki Umezawa², Yasumichi Inoue², Isamu Akiba³, Takehisa Dewa^{1

4}, Atsushi Ikeda⁵, Toshihisa Mizuno^{1

4}

Affiliations

¹ Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi 466-8555, Japan. mizuno.toshihisa@nitech.ac.jp.
² Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan.
³ Faculty of Environmental Engineering, the University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, Fukuoka 808-0135, Japan.
⁴ Department of Nanopharmaceutical Sciences, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi 466-8555, Japan.
⁵ Applied Chemistry Program, Graduate School of Advanced Science and Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan.

PMID: 38165681
DOI: 10.1039/d3cc05347d

Abstract

This study explores a new method for delivering therapeutic proteins into specific cells using OLE-ZIP capsules that present IgG. OLE-ZIP capsules is a spherical caspules prepared from amphihilic dimetic coiled-coil peptide, OLE-ZIP. Upon presenting cetuximab, these capsules showed preferential uptake in A431 cells and increased cytotoxicity when loaded with RNase A.

MeSH terms

Cytoplasm
Immunoglobulin G*
Peptides*

Substances

Peptides
Immunoglobulin G