Standardizing Minimally Invasive Tissue Sampling of Postmortem Brain Using Bard Monopty Needle in Newborns with Neurological Injury

Fetal Pediatr Pathol. 2024 Mar-Apr;43(2):94-110. doi: 10.1080/15513815.2023.2301448. Epub 2024 Jan 10.

Abstract

Introduction: Minimally invasive tissue sampling of the brain in newborns using the Bard Monopty needle helps to diagnose various neurological conditions by obtaining relevant brain cores. We designed a modified procedure to provide maximum diagnostic utility in brain tissue biopsies.

Method: Twenty newborns underwent postmortem minimally invasive tissue sampling of the brain through the anterior fontanelle and posterior approach, using the engraved lines on the needle labeled from mark 0 to 13. The cores were correlated with conventional autopsy findings.

Results: Meninges were best obtained at marks 0 and 1 from the anterior fontanelle and mark 1 from posterior fontenelle in 85% of cases. Periventricular brain parenchyma was best obtained from mark 3 and mark 1 from anterior and posterior fontanel, respectively in 90% cases. The sampling success in obtaining brain cores was 100%.

Discussion: This modified technique increases the yield of meninges and brain tissue in newborns and aids in diagnosis.

Keywords: Autopsy; Bard Monopty needle; brain; minimally invasive tissue sampling; neonate.

MeSH terms

  • Autopsy / methods
  • Biopsy
  • Brain*
  • Humans
  • Infant, Newborn
  • Needles*