An improved pathway for autonomous bioluminescence imaging in eukaryotes

Ekaterina S Shakhova; Tatiana A Karataeva; Nadezhda M Markina; Tatiana Mitiouchkina; Kseniia A Palkina; Maxim M Perfilov; Monika G Wood; Trish T Hoang; Mary P Hall; Liliia I Fakhranurova; Anna E Alekberova; Alena K Malyshevskaia; Dmitry A Gorbachev; Evgenia N Bugaeva; Ludmila K Pletneva; Vladislav V Babenko; Daria I Boldyreva; Andrey Y Gorokhovatsky; Anastasia V Balakireva; Feng Gao; Vladimir V Choob; Lance P Encell; Keith V Wood; Ilia V Yampolsky; Karen S Sarkisyan; Alexander S Mishin

doi:10.1038/s41592-023-02152-y

An improved pathway for autonomous bioluminescence imaging in eukaryotes

Nat Methods. 2024 Mar;21(3):406-410. doi: 10.1038/s41592-023-02152-y. Epub 2024 Jan 22.

Authors

Ekaterina S Shakhova^#^{1

2}, Tatiana A Karataeva^#^{1

2}, Nadezhda M Markina^#^{1

2}, Tatiana Mitiouchkina^#^{1

2}, Kseniia A Palkina^#^{1

2}, Maxim M Perfilov^#^{1

2}, Monika G Wood³, Trish T Hoang³, Mary P Hall³, Liliia I Fakhranurova¹, Anna E Alekberova², Alena K Malyshevskaia^{1

2}, Dmitry A Gorbachev^{1

2}, Evgenia N Bugaeva¹, Ludmila K Pletneva¹, Vladislav V Babenko⁴, Daria I Boldyreva⁴, Andrey Y Gorokhovatsky², Anastasia V Balakireva^{1

2}, Feng Gao^{5

6}, Vladimir V Choob^{1

7}, Lance P Encell³, Keith V Wood⁸, Ilia V Yampolsky^{1

2

8

9}, Karen S Sarkisyan^{10

11

12

13

14}, Alexander S Mishin^{15

16}

Affiliations

¹ Planta LLC, Moscow, Russia.
² Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
³ Promega Corporation, Madison, WI, USA.
⁴ Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.
⁵ Synthetic Biology Group, MRC Laboratory of Medical Sciences, London, UK.
⁶ Institute of Clinical Sciences, Faculty of Medicine and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK.
⁷ Botanical Garden of Lomonosov Moscow State University, Moscow, Russia.
⁸ Light Bio Inc, Ketchum, ID, USA.
⁹ Pirogov Russian National Research Medical University, Moscow, Russia.
¹⁰ Planta LLC, Moscow, Russia. karen@light.bio.
¹¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. karen@light.bio.
¹² Synthetic Biology Group, MRC Laboratory of Medical Sciences, London, UK. karen@light.bio.
¹³ Institute of Clinical Sciences, Faculty of Medicine and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK. karen@light.bio.
¹⁴ Light Bio Inc, Ketchum, ID, USA. karen@light.bio.
¹⁵ Planta LLC, Moscow, Russia. alexander@planta.bio.
¹⁶ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. alexander@planta.bio.

^# Contributed equally.

Abstract

The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.

MeSH terms

Animals
Eukaryota*
Luminescence*
Mammals

Abstract

MeSH terms

Grants and funding