Antimutagenic and antitumor activities of a water-soluble fraction of soursop (syn Graviola, Annona muricata L.) fruit pulp

J Toxicol Environ Health A. 2024 Apr 2;87(7):310-324. doi: 10.1080/15287394.2024.2309335. Epub 2024 Feb 5.

Abstract

Soursop (Annona muricata) is a tropical tree whose decoction derived from bark, root, seed, or leaf has been used for medicinal uses. In addition, the fruit itself is considered a food, and the juice is utilized to treat heart and liver diseases. The aim of this study was to determine the phenolic content. In addition, a water-soluble fraction of the soursop fruit pulp (WSSP) was examined for the following properties: antioxidant, mutagenic, and antimutagenicity. UV-visible spectrophotometry determined total phenolic content by the Folin-Ciocalteu method to be 11.22 ± 0.6 mg of gallic acid equivalent per gram dried extract, and free-radical scavenging activity by the 2,2'-diphenyl-1-picryl-hydrazyl (DPPH•) showed an EC50 of 1032 µg/ml. In the Salmonella/microsome assay, no marked mutagenicity was induced following WSSP treatment, and a chemopreventive capacity was observed in the antimutagenic assay. The cytotoxicity assays were carried out using the water-soluble tetrazolium salt and lactate dehydrogenase (LDH) assays demonstrated that WSSP induced significant cytotoxicity in MCF-7 and Caco-2 cells, indicating greater effectiveness of cytotoxic action by destroying cell membrane integrity. Data suggest that WSSP may exert beneficial effects as a DNA chemopreventive and antitumor agent.

Keywords: Annona muricata; antimutagenicity; antioxidant; antitumor activity; phenolic content.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Annona* / chemistry
  • Antioxidants / pharmacology
  • Caco-2 Cells
  • Fruit / chemistry
  • Humans
  • Phenols / analysis
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology

Substances

  • Plant Extracts
  • Phenols
  • Antioxidants