ABHD7-mediated depalmitoylation of lamin A promotes myoblast differentiation

Yuan Shen; Liang-Liang Zheng; Cai-Yun Fang; Yao-Yao Xu; Chao Wang; Jin-Tao Li; Ming-Zhu Lei; Miao Yin; Hao-Jie Lu; Qun-Ying Lei; Jia Qu

doi:10.1016/j.celrep.2024.113720

ABHD7-mediated depalmitoylation of lamin A promotes myoblast differentiation

Cell Rep. 2024 Feb 27;43(2):113720. doi: 10.1016/j.celrep.2024.113720. Epub 2024 Feb 2.

Authors

Yuan Shen¹, Liang-Liang Zheng¹, Cai-Yun Fang², Yao-Yao Xu³, Chao Wang¹, Jin-Tao Li¹, Ming-Zhu Lei¹, Miao Yin¹, Hao-Jie Lu⁴, Qun-Ying Lei⁵, Jia Qu⁶

Affiliations

¹ Fudan University Shanghai Cancer Center & Institutes of Biomedical Sciences, Cancer Institutes, Key Laboratory of Breast Cancer in Shanghai, Shanghai Key Laboratory of Radiation Oncology, The Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
² Department of Chemistry, Fudan University, Shanghai 200438, China.
³ Fudan University Shanghai Cancer Center & Institutes of Biomedical Sciences, Cancer Institutes, Key Laboratory of Breast Cancer in Shanghai, Shanghai Key Laboratory of Radiation Oncology, The Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University, Shanghai 200032, China.
⁴ Fudan University Shanghai Cancer Center & Institutes of Biomedical Sciences, Cancer Institutes, Key Laboratory of Breast Cancer in Shanghai, Shanghai Key Laboratory of Radiation Oncology, The Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University, Shanghai 200032, China; NHC Key Laboratory of Glycoconjugates Research, Fudan University, Shanghai 200032, China; Department of Chemistry, Fudan University, Shanghai 200438, China. Electronic address: luhaojie@fudan.edu.cn.
⁵ Fudan University Shanghai Cancer Center & Institutes of Biomedical Sciences, Cancer Institutes, Key Laboratory of Breast Cancer in Shanghai, Shanghai Key Laboratory of Radiation Oncology, The Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China; State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China; New Cornerstone Science Laboratory, Fudan University, Shanghai 200032, China. Electronic address: qlei@fudan.edu.cn.
⁶ Fudan University Shanghai Cancer Center & Institutes of Biomedical Sciences, Cancer Institutes, Key Laboratory of Breast Cancer in Shanghai, Shanghai Key Laboratory of Radiation Oncology, The Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. Electronic address: jqu@fudan.edu.cn.

PMID: 38308845
DOI: 10.1016/j.celrep.2024.113720

Abstract

LMNA gene mutation can cause muscular dystrophy, and post-translational modification plays a critical role in regulating its function. Here, we identify that lamin A is palmitoylated at cysteine 522, 588, and 591 residues, which are reversely catalyzed by palmitoyltransferase zinc finger DHHC-type palmitoyltransferase 5 (ZDHHC5) and depalmitoylase α/β hydrolase domain 7 (ABHD7). Furthermore, the metabolite lactate promotes palmitoylation of lamin A by inhibiting the interaction between it and ABHD7. Interestingly, low-level palmitoylation of lamin A promotes, whereas high-level palmitoylation of lamin A inhibits, murine myoblast differentiation. Together, these observations suggest that ABHD7-mediated depalmitoylation of lamin A controls myoblast differentiation.

Keywords: ABHD7; CP: Molecular biology; CP: Stem cell research; ZDHHC5; lactate; lamin A; myoblast differentiation; palmitoylation.

MeSH terms

Animals
Cell Differentiation
Lamin Type A* / metabolism
Mice
Muscular Dystrophies* / genetics
Myoblasts / metabolism
Protein Processing, Post-Translational

Substances

Lamin Type A
Lmna protein, mouse