Correlates of Rotavirus Vaccine Shedding and Seroconversion in a US Cohort of Healthy Infants

J Infect Dis. 2024 Sep 23;230(3):754-762. doi: 10.1093/infdis/jiae055.

Abstract

Background: Rotavirus is a leading cause of severe pediatric gastroenteritis; 2 highly effective vaccines are used in the United States (US). We aimed to identify correlates of immune response to rotavirus vaccination in a US cohort.

Methods: Pediatric Respiratory and Enteric Virus Acquisition and Immunogenesis Longitudinal (PREVAIL) is a birth cohort of 245 mother-child pairs enrolled in 2017-2018 and followed for 2 years. Infant stool samples and symptom information were collected weekly. Shedding was defined as reverse-transcription polymerase chain reaction detection of rotavirus vaccine virus in stools collected 4-28 days after dose 1. Seroconversion was defined as a 3-fold rise in immunoglobulin A between the 6-week and 6-month blood draws. Correlates were analyzed using generalized estimating equations and logistic regression.

Results: Prevaccination immunoglobulin G (IgG) (odds ratio [OR], 0.84 [95% confidence interval {CI}, .75-.94] per 100-unit increase) was negatively associated with shedding. Shedding was also less likely among infants with a single-nucleotide polymorphism inactivating FUT2 antigen secretion ("nonsecretors") with nonsecretor mothers, versus all other combinations (OR, 0.37 [95% CI, .16-.83]). Of 141 infants with data, 105 (74%) seroconverted; 78 (77%) had shed vaccine virus following dose 1. Prevaccination IgG and secretor status were significantly associated with seroconversion. Neither shedding nor seroconversion significantly differed by vaccine product.

Conclusions: In this US cohort, prevaccination IgG and maternal and infant secretor status were associated with rotavirus vaccine response.

Keywords: histo-blood group antigens (HBGA); pediatric gastroenteritis; rotavirus; rotavirus vaccine; vaccine immunogenicity; vaccine response; vaccine shedding.

MeSH terms

  • Adult
  • Antibodies, Viral* / blood
  • Birth Cohort
  • Cohort Studies
  • Feces* / virology
  • Female
  • Humans
  • Immunoglobulin A / blood
  • Immunoglobulin A / immunology
  • Immunoglobulin G* / blood
  • Immunoglobulin G* / immunology
  • Infant
  • Longitudinal Studies
  • Male
  • Rotavirus Infections* / immunology
  • Rotavirus Infections* / prevention & control
  • Rotavirus Vaccines* / administration & dosage
  • Rotavirus Vaccines* / immunology
  • Rotavirus* / immunology
  • Seroconversion*
  • United States
  • Vaccination
  • Virus Shedding*

Substances

  • Rotavirus Vaccines
  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin A