Clinical relevance of doubtful reactions in patch testing: A single-centre retrospective study

Contact Dermatitis. 2024 Jun;90(6):607-612. doi: 10.1111/cod.14526. Epub 2024 Feb 21.

Abstract

Background: Doubtful reactions in patch testing are infrequently reported in the literature; however, recent reports have suggested they be assessed with the same scrutiny as stronger reactions.

Objective: Assess the clinical relevance of doubtful reactions in patch testing.

Methods: Retrospective study of 1514 patients comprehensively patch tested via the NACDG standard series and additional allergens based on history. The clinical relevance of each reaction was graded based on the NACDG scale: definite, probable, possible, past, unknown and irritant. Reactions were considered 'unique' if an additional mild-to-strong reaction to the same chemical at a different concentration was not observed.

Results: 68.9% (1043) of patients demonstrated at least 1 doubtful reaction. Of 4453 total doubtful reactions, 92.2% (4106) were unique. Only 3.3% (137) and 12.2% (500) of these were determined to be of definite or probable clinical relevance respectively. 'Fragrance' was the most common allergen family present among the unique definite doubtful reactions (37). However, 24 (64.9%) of these also had a stronger reaction to another fragrance. Cocamidopropyl betaine was the second most frequent allergen demonstrating definite doubtful reactions (27) and unique in 85.2% (23) of cases. Methylchloroisothiazolinone/methylisothiazolinone (MCI/MI) was most prevalent (36) but less frequently unique (58.3%, 21).

Conclusions: Doubtful reactions may not be as impactful to clinical decision making as theorised in the literature. Few demonstrate definite clinical significance, and many have related stronger reactions that capture them for clinical purposes. Identification of doubtful reactions to cocamidopropyl betaine and MCI/MI may be of greatest significance as they most frequently were not supported by stronger reactions.

Keywords: allergic contact dermatitis; borderline reaction; clinical relevance; doubtful reaction; macular erythema; patch test.

MeSH terms

  • Adult
  • Allergens* / adverse effects
  • Clinical Relevance
  • Dermatitis, Allergic Contact* / diagnosis
  • Dermatitis, Allergic Contact* / etiology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Patch Tests* / methods
  • Perfume / adverse effects
  • Retrospective Studies

Substances

  • Allergens
  • Perfume