[Mechanism about LMP1 of EB Virus Promoting Plasma Blast Differentiation of DLBCL Cell via mTORC1]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2024 Feb;32(1):219-224. doi: 10.19746/j.cnki.issn.1009-2137.2024.01.035.
[Article in Chinese]

Abstract
in English, Chinese

Objective: To investigate possible mechanism on protien LMP1 expressed by EBV inducing plasmablast differentiation of DLBCL cell via the mTORC1 pathway.

Methods: The expression levels of LMP1 protein, CD38 and the phosphorylation levels of p70S6K in EBV+ and EBV- DLBCL cell lines were detected by Western blot. Cell lines overexpressing LMP1 gene stablely were constructed and LMP1 gene was silenced by RNAi. The expression of LMP1 gene was verified by RT-qPCR. The expression levels of LMP1 and CD38 and the phosphorylation levels of p70S6K in each group were detected by Western blot.

Results: Compared with EBV-DLBCL cells, the expression of LMP1 was detected on EBV +DLBCL cells (P =0.0008), EBV +DLBCL cells had higher phosphorylation levels of p70S6K (P =0.0072) and expression levels of CD38(P =0.0091). Compared with vector group, the cells of LMP1OE group had higher expression levels of LMP1 and CD38 (P =0.0353; P <0.0001), meanwhile molecular p70S6K was phosphorylated much more(P =0.0065); expression of LMP1 mRNA was verified(P <0.0001). Compared with si-NC group, expression level of LMP1 protein(P =0.0129) was not detected and phosphorylated p70S6K disappeared of LMP1KO group (P =0.0228); meanwhile, expression of CD38 decreased,although there was no significant difference (P =0.2377).

Conclusion: LMP1 promotes DLBCL cells plasmablast differentiation via activating mTORC1 signal pathway.

题目: mTORC1在EB病毒LMP1促进DLBCL细胞母细胞化的机制研究.

目的: 研究通过mTORC1通路EB病毒LMP1诱导弥漫大B细胞淋巴瘤（DLBCL）细胞母细胞化的可能机制。.

方法: 采用Western blot法分析EBV+及EBV-DLBCL细胞株LMP1蛋白、CD38的表达及p70S6K磷酸化情况。构建过表达LMP1稳转株及RNAi沉默LMP1基因，用RT-qPCR验证基因表达，并利用Western blot法检测各组细胞LMP1蛋白、CD38的表达量及较EBV- p70S6K磷酸化水平。.

结果: 相较于EBV- DLBCL细胞，LMP1蛋白在EBV+DLBCL细胞上表达（P =0.0008），EBV+DLBCL细胞p70S6K磷酸化水平更高（P =0.0072）及CD38的表达量更高（P =0.0091）。与空载组对比，LMP1OE组的LMP1蛋白表达及CD38表达量均增高（P =0.0353; P < 0.0001），且p70S6K磷酸化水平增高（P =0.0065）；并验证了LMP1 mRNA表达（P < 0.0001）。较si-NC组，LMP1KO组不表达LMP1蛋白（P =0.0129），且p70S6K磷酸化消失（P =0.0228）；同时，CD38表达量减少，但无显著性差异（P =0.2377）。.

结论: LMP1通过活化mTORC1通路促进DLBCL细胞浆母细胞化。.

Keywords: LMP1; CD38; DLBCL; EB virus; mTORC1 pathway; plasmablast differentiation.

Publication types

  • English Abstract

MeSH terms

  • Cell Line
  • Herpesvirus 4, Human*
  • Humans
  • Mechanistic Target of Rapamycin Complex 1 / metabolism
  • Ribosomal Protein S6 Kinases, 70-kDa* / metabolism
  • Signal Transduction
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / metabolism

Substances

  • Ribosomal Protein S6 Kinases, 70-kDa
  • Mechanistic Target of Rapamycin Complex 1
  • Viral Matrix Proteins