Octadecyl Gallate and Lipid-Modified MnSe2 Nanoparticles Enhance Radiosensitivity in Esophageal Squamous Cell Carcinoma and Promote Radioprotection in Normal Tissues

Adv Mater. 2024 Jun;36(23):e2311291. doi: 10.1002/adma.202311291. Epub 2024 Mar 12.

Abstract

Radiotherapy, a widely used therapeutic strategy for esophageal squamous cell carcinoma (ESCC), is always limited by radioresistance of tumor tissues and side-effects on normal tissues. Herein, a signature based on four core genes of cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway, is developed to predict prognosis and assess immune cell infiltration, indicating that the cGAS-STING pathway and radiotherapy efficacy are closely intertwined in ESCC. A novel lipid-modified manganese diselenide nanoparticle (MnSe2-lipid) with extraordinarily uniform sphere morphology and tumor microenvironment (TME) responsiveness is developed to simultaneously overcome radioresistance and reduce side-effects of radiation. The uniform MnSe2 encapsulated lipid effectively achieves tumor accumulation. Octadecyl gallate on surface of MnSe2 forming pH-responsive metal-phenolic covalent realizes rapid degradation in TME. The released Mn2+ promotes radiosensitivity by generating reactive oxygen species induced by Fenton-like reaction and activating cGAS-STING pathway. Spontaneously, selenium strengthens immune response by promoting secretion of cytokines and increasing white blood cells, and performs antioxidant activity to reduce side-effects of radiotherapy. Overall, this multifunctional remedy which is responsive to TME is capable of providing radiosensitivity by cGAS-STING pathway-mediated immunostimulation and chemodynamic therapy, and radioprotection of normal tissues, is highlighted here to optimize ESCC treatment.

Keywords: MnSe2‐lipid; cyclic GMP‐AMP synthase‐stimulator of interferon genes; esophageal squamous cell carcinoma; radioprotection; radiosensitivity.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Esophageal Neoplasms* / drug therapy
  • Esophageal Neoplasms* / metabolism
  • Esophageal Neoplasms* / pathology
  • Esophageal Squamous Cell Carcinoma* / drug therapy
  • Esophageal Squamous Cell Carcinoma* / pathology
  • Gallic Acid / analogs & derivatives
  • Gallic Acid / chemistry
  • Gallic Acid / pharmacology
  • Humans
  • Lipids / chemistry
  • Manganese / chemistry
  • Mice
  • Nanoparticles* / chemistry
  • Radiation Tolerance* / drug effects
  • Radiation-Protective Agents / chemistry
  • Radiation-Protective Agents / pharmacology
  • Radiation-Sensitizing Agents / chemistry
  • Radiation-Sensitizing Agents / pharmacology
  • Selenium / chemistry
  • Selenium / pharmacology
  • Tumor Microenvironment / drug effects

Substances

  • Gallic Acid
  • Lipids
  • Selenium
  • Radiation-Protective Agents
  • Manganese
  • Radiation-Sensitizing Agents