Identification of macaque dendritic cell precursors in blood and tissue reveals their dysregulation in early SIV infection

Cell Rep. 2024 Apr 23;43(4):113994. doi: 10.1016/j.celrep.2024.113994. Epub 2024 Mar 25.

Abstract

Distinct dendritic cell (DC) subsets play important roles in shaping immune responses. Circulating DC precursors (pre-DCs) are more susceptible to HIV infection in vitro, which may explain the inefficiency of immune responses against HIV. However, the interplay between HIV and pre-DC is not defined in vivo. We identify human pre-DC equivalents in the cynomolgus macaque and then analyze their dynamics during simian immunodeficiency virus (SIV) infection to illustrate a sharp decrease of blood pre-DCs in early SIV infection and accumulation in lymph nodes (LNs), where they neglect to upregulate CD83/CD86 or MHC-II. Additionally, SIV infection attenuates the capacity of stimulated LN pre-DCs to produce IL-12p40. Analysis of HIV cohorts provides correlation between costimulatory molecule expression on pre-DCs and T cell activation in spontaneous HIV controllers. These findings pinpoint certain dynamics and functional changes of pre-DCs during SIV infection, providing a deeper understanding of immune dysregulation mechanisms elicited in people living with HIV.

Keywords: CP: Immunology; HIV; HIV controllers; SIV; anti-viral immune responses; cDC; cynomolgus macaques; immune dysregulation; lymph nodes; pDC; pre-DC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Dendritic Cells* / immunology
  • HIV Infections / blood
  • HIV Infections / immunology
  • HIV Infections / pathology
  • HIV Infections / virology
  • Humans
  • Lymph Nodes / immunology
  • Lymph Nodes / pathology
  • Lymphocyte Activation / immunology
  • Macaca fascicularis
  • Simian Acquired Immunodeficiency Syndrome* / blood
  • Simian Acquired Immunodeficiency Syndrome* / immunology
  • Simian Acquired Immunodeficiency Syndrome* / pathology
  • Simian Acquired Immunodeficiency Syndrome* / virology
  • Simian Immunodeficiency Virus* / immunology