Cytokines-activated nuclear IKKα-FAT10 pathway induces breast cancer tamoxifen-resistance

Sci China Life Sci. 2024 Jul;67(7):1413-1426. doi: 10.1007/s11427-023-2460-0. Epub 2024 Apr 1.

Abstract

Endocrine therapy that blocks estrogen signaling is the most effective treatment for patients with estrogen receptor positive (ER+) breast cancer. However, the efficacy of agents such as tamoxifen (Tam) is often compromised by the development of resistance. Here we report that cytokines-activated nuclear IKKα confers Tam resistance to ER+ breast cancer by inducing the expression of FAT10, and that the expression of FAT10 and nuclear IKKα in primary ER+ human breast cancer was correlated with lymphotoxin β (LTB) expression and significantly associated with relapse and metastasis in patients treated with adjuvant mono-Tam. IKKα activation or enforced FAT10 expression promotes Tam-resistance while loss of IKKα or FAT10 augments Tam sensitivity. The induction of FAT10 by IKKα is mediated by the transcription factor Pax5, and coordinated via an IKKα-p53-miR-23a circuit in which activation of IKKα attenuates p53-directed repression of FAT10. Thus, our findings establish IKKα-to-FAT10 pathway as a new therapeutic target for the treatment of Tam-resistant ER+ breast cancer.

Keywords: FAT10; Pax5; Tam-resistance; breast cancer; lymphotoxin; nuclear IKKα; nuclear IKKα-FAT0 pathway.

MeSH terms

  • Animals
  • Antineoplastic Agents, Hormonal / pharmacology
  • Antineoplastic Agents, Hormonal / therapeutic use
  • Breast Neoplasms* / drug therapy
  • Breast Neoplasms* / genetics
  • Breast Neoplasms* / metabolism
  • Cell Line, Tumor
  • Cytokines / metabolism
  • Drug Resistance, Neoplasm* / genetics
  • Female
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • I-kappa B Kinase* / metabolism
  • MCF-7 Cells
  • Signal Transduction* / drug effects
  • Tamoxifen* / pharmacology
  • Tamoxifen* / therapeutic use
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism

Substances

  • Antineoplastic Agents, Hormonal
  • CHUK protein, human
  • Cytokines
  • I-kappa B Kinase
  • Tamoxifen
  • Tumor Suppressor Protein p53
  • UBD protein, human