Carrying SNP rs17506395 (T > G) in TP63 gene and CCR5Δ32 mutation associated with the occurrence of breast cancer in Burkina Faso

Lassina Traoré; Mousso Savadogo; Abdou Azaque Zouré; Touwendpoulimdé Isabelle Kiendrebeogo; Fabienne Marie B T B Soudre; Soayebo Dabre; Aida Djé Djénéba Traore; Marc Donald Wilfried Adico; Tilate Lare; Teega-Wendé Clarisse Ouedraogo; Rogomenoma Alice Ouedraogo; Abdoul Karim Ouattara; Edwige T Yelemkoure; Alexis Yobi Sawadogo; Nayi Zongo; Hierrhum Aboubacar Bambara; Christelle W Nadembega; Florencia W Djigma; Jacques Simpore

doi:10.1515/biol-2022-0847

Carrying SNP rs17506395 (T > G) in TP63 gene and CCR5Δ32 mutation associated with the occurrence of breast cancer in Burkina Faso

Open Life Sci. 2024 Apr 3;19(1):20220847. doi: 10.1515/biol-2022-0847. eCollection 2024.

Authors

Lassina Traoré^{1

2}, Mousso Savadogo^{1

2}, Abdou Azaque Zouré^{1

2

3}, Touwendpoulimdé Isabelle Kiendrebeogo^{1

2}, Fabienne Marie B T B Soudre^{1

4}, Soayebo Dabre^{1

2}, Aida Djé Djénéba Traore^{1

2}, Marc Donald Wilfried Adico^{1

2}, Tilate Lare^{1

2}, Teega-Wendé Clarisse Ouedraogo^{1

2}, Rogomenoma Alice Ouedraogo^{1

2

5}, Abdoul Karim Ouattara^{1

2

6}, Edwige T Yelemkoure^{1

2}, Alexis Yobi Sawadogo⁷, Nayi Zongo⁸, Hierrhum Aboubacar Bambara⁹, Christelle W Nadembega^{1

2}, Florencia W Djigma^{1

2}, Jacques Simpore^{1

2}

Affiliations

¹ Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, 03 BP 7021, Ouagadougou 03, Burkina Faso.
² Pietro Annigoni Biomolecular Research Centre (CERBA), 01 BP 364, Ouagadougou 01, Burkina Faso.
³ Biomedical Research Laboratory (LaReBio), Biomedical and Public Health Department, Health Sciences Research Institute (IRSS/CNRST), 03 BP 7192 Ouaga 03, Burkina Faso.
⁴ Laboratory Department, University Hospital Centre-Yalgado OUEDRAOGO, Joseph KI-ZERBO University, UFR/SDS, 03 BP 7021, Ouagadougou 03, Ouagadougou, Burkina Faso.
⁵ Gaoua University Centre, NAZI BONI University, 01 BP 1091, Bobo-Dioulasso 01, Burkina Faso.
⁶ Manga University Centre, Norbert ZONGO University, Koudougou, Burkina Faso.
⁷ Gynecology Department, Yalgado Ouédraogo University Hospital, UFR/SDS, 03 BP 7021, Ouagadougou 03, Ouagadougou, Burkina Faso.
⁸ Department of Surgery, Visceral and Digestive Surgery Service, Yalgado Ouédraogo University Hospital, Joseph KI-ZERBO University, UFR/SDS 03 BP 7021, Ouagadougou 03, Ouagadougou, Burkina Faso.
⁹ Oncology Department, University Hospital Centre-BOGODOGO, Joseph KI-ZERBO University, UFR/SDS, 03 BP 7021, Ouagadougou 03, Ouagadougou, Burkina Faso.

Abstract

Genetic alterations in the TP63 (GenBank: NC_000003.12, ID: 8626) and CCR5 (receptor 5 chemokine co-receptor) (GenBank: NC_000003.12, ID: 1234) genes may increase the risk of developing breast cancer. The aim of this study was to investigate the probable involvement of polymorphisms rs17506395 in the TP63 (tumour protein 63) gene and the CCR5Δ32 mutation in the occurrence of breast cancer in Burkina Faso. This case-control study included 72 patients and 72 controls. Genotyping of SNP rs17506395 (TP63) was performed by polymerase chain reaction-restriction fragment length polymorphism, and genotyping of the CCR5Δ32 mutation was performed by allele-specific oligonucleotide polymerase chain reaction. For SNP rs17506395 (TP63), the genotypic frequencies of wild-type homozygotes (TT) and heterozygotes (TG) were, respectively, 27.72 and 72.22% in cases and 36.11 and 63.89% in controls. No mutated homozygotes (GG) were observed. For the CCR5Δ32 mutation, the genotypic frequencies of wild-type homozygotes (WT/WT) and heterozygotes (WT/Δ32) were 87.5 and 13.5%, respectively, in the cases and 89.29 and 10.71%, respectively, in the controls. No mutated homozygotes (Δ32/Δ32) were observed. None of the polymorphisms rs17506395 of the TP63 gene (OR = 1.47, 95% CI = 0.69-3.17, P = 0.284) and the CCR5Δ32 mutation (OR = 1.32, 95% CI = 0.46-3.77; P = 0.79) were associated with the occurrence of breast cancer in this study.

Keywords: Burkina Faso; CCR5; TP63; breast cancer; polymorphism.