Fungal microbiota in newborn infants with and without respiratory distress syndrome

PLoS One. 2024 Apr 10;19(4):e0302027. doi: 10.1371/journal.pone.0302027. eCollection 2024.

Abstract

Background: Pneumocytis jirovecii infection in preterm newborns has recently been associated with neonatal respiratory distress syndrome and bronchopulmonary dysplasia. Changes in the bacterial microbiota of the airways have also been described in infants with bronchopulmonary dysplasia. However, until now there has been no information on the airway mycobiota in newborns. The purpose of this study was to describe the airway mycobiota in term and preterm newborns and its possible association with respiratory distress syndrome.

Methods: Twenty-six matched preterm newborns with and without respiratory distress syndrome were studied, as well as 13 term babies. The identification of the fungal microbiota was carried out using molecular procedures in aspirated nasal samples at birth.

Results: The ascomycota phylum was identified in 89.7% of newborns, while the basidiomycota phylum was found in 33.3%. Cladosporium was the predominant genus in both term and preterm infants 38.4% vs. 73% without statistical differences. Candida sake and Pneumocystis jirovecii were only found in preterm infants, suggesting a potential relationship with the risk of prematurity.

Conclusions: This is the first report to describe the fungal microbiota of the airways in term and preterm infants with and without respiratory distress syndrome. Although no differences have been observed, the number of cases analyzed could be small to obtain conclusive results, and more studies are needed to understand the role of the fungal microbiota of the airways in neonatal respiratory pathology.

MeSH terms

  • Bronchopulmonary Dysplasia*
  • Humans
  • Infant
  • Infant, Newborn
  • Infant, Premature
  • Mycobiome*
  • Pneumocystis carinii*
  • Respiratory Distress Syndrome, Newborn*

Grants and funding

“Institute of Health Carlos III, Spanish Ministry of Science, Innovation and University [Grant number: FIS-PI19/01845] and by European Regional Development Fund (ERDF) and the Ministry of Economic Transformation, Industry, Knowledge and Universities of the Junta de Andalucía, within the ERDF Operational Program 2014-2020" [FEDER-US-1381054]. Carmen de la Horra and Rocío Salsoso were supported by the postdoctoral contract programme of the Consejería de Salud y Consumo [CS 2021 POSTDOC] and the Consejería de Universidad, Investigación, and Innovación [POSTDOC_21_00907], respectively”.