Crosstalk between endothelial cells and dermal papilla entails hair regeneration and angiogenesis during aging

Siyi Zhou; Zeming Li; Xinzhu Li; Yuanli Ye; Mengyue Wang; Jingwei Jiang; Lihe Tao; Yan Wang; Chen-Tsen Tung; Yongji Chung; Eunmi Kim; Xinyu Shen; Xuegang Xu; Xiao Xiang; Qiaoli Xie; Jinwei Zhang; Wang Wu; Xia Lin; Cheng-Ming Chuong; Mingxing Lei

doi:10.1016/j.jare.2024.05.006

Crosstalk between endothelial cells and dermal papilla entails hair regeneration and angiogenesis during aging

J Adv Res. 2024 May 7:S2090-1232(24)00183-8. doi: 10.1016/j.jare.2024.05.006. Online ahead of print.

Authors

Siyi Zhou¹, Zeming Li², Xinzhu Li³, Yuanli Ye⁴, Mengyue Wang⁵, Jingwei Jiang⁶, Lihe Tao⁷, Yan Wang⁸, Chen-Tsen Tung⁹, Yongji Chung¹⁰, Eunmi Kim¹¹, Xinyu Shen¹², Xuegang Xu¹³, Xiao Xiang¹⁴, Qiaoli Xie¹⁵, Jinwei Zhang¹⁶, Wang Wu¹⁷, Xia Lin¹⁸, Cheng-Ming Chuong¹⁹, Mingxing Lei²⁰

Affiliations

¹ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: 202119021085@stu.cqu.edu.cn.
² Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: 202219021036@stu.cqu.edu.cn.
³ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: 20221901001z@stu.cqu.edu.cn.
⁴ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: 202319021075T@stu.cqu.edu.cn.
⁵ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: Wmy2017@cqu.edu.cn.
⁶ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: 20201901033@stu.cqu.edu.cn.
⁷ Zhejiang Keyikaijian Bioengineering Co., Ltd, Beijing 100191, China. Electronic address: tlhmichael@dermaheal.com.cn.
⁸ Zhejiang Keyikaijian Bioengineering Co., Ltd, Beijing 100191, China. Electronic address: wangyan@dermaheal.com.cn.
⁹ Zhejiang Keyikaijian Bioengineering Co., Ltd, Beijing 100191, China. Electronic address: r_t1969@163.com.
¹⁰ Caregen Co., Ltd, Gyeonggi-do 14119, Korea. Electronic address: chung@caregen.co.kr.
¹¹ Caregen Co., Ltd, Gyeonggi-do 14119, Korea. Electronic address: dmsal@caregen.co.kr.
¹² Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: 202219131160@stu.cqu.edu.cn.
¹³ Department of Dermatology, The First Hospital of China Medical University NHC Key Laboratory of Immunodermatology (China Medical University), Key Laboratory of Immunodermatology (China Medical University), Shenyang, Liaoning Province 110001, China. Electronic address: xuxuegang2749290@163.com.
¹⁴ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: xiaoxiang_bioeng@cqu.edu.cn.
¹⁵ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: qiaolixie@cqu.edu.cn.
¹⁶ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: skinzjw@163.com.
¹⁷ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: wwang@cqu.edu.cn.
¹⁸ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: 249239625@qq.com.
¹⁹ Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. Electronic address: cmchuong@usc.edu.
²⁰ Key Laboratory of Biorheological Science and Technology of Ministry of Education & 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: mingxing@cqu.edu.cn.

PMID: 38718895
DOI: 10.1016/j.jare.2024.05.006

Abstract

Introduction: Tissues maintain their function through interaction with microenvironment. During aging, both hair follicles and blood vessels (BV) in skin undergo degenerative changes. However, it is elusive whether the changes are due to intrinsic aging changes in hair follicles or blood vessels respectively, or their interactions.

Objective: To explore how hair follicles and blood vessels interact to regulate angiogenesis and hair regeneration during aging.

Methods: Single-cell RNA-sequencing (scRNA-seq) analyses were used to identify the declined ability of dermal papilla (DP) and endothelial cells (ECs) during aging. CellChat and CellCall were performed to investigate interaction between DP and ECs. Single-cell metabolism (scMetabolism) analysis and iPATH were applied to analyze downstream metabolites in DP and ECs. Hair-plucking model and mouse cell organoid model were used for functional studies.

Results: During aging, distance and interaction between DP and ECs are decreased. DP interacts with ECs, with decreased EDN1-EDNRA signaling from ECs to DP and CTF1-IL6ST signaling from DP to ECs during aging. ECs-secreted EDN1 binds to DP-expressed EDNRA which enhances Taurine (TA) metabolism to promote hair regeneration. DP-emitted CTF1 binds to ECs-expressed IL6ST which activates alpha-linolenic acid (ALA) metabolism to promote angiogenesis. Activated EDN1-EDNRA-TA signaling promotes hair regeneration in aged mouse skin and in organoid cultures, and increased CTF1-IL6ST-ALA signaling also promotes angiogenesis in aged mouse skin and organoid cultures.

Conclusions: Our finding reveals reciprocal interactions between ECs and DP. ECs releases EDN1 sensed by DP to activate TA metabolism which induces hair regeneration, while DP emits CTF1 signal received by ECs to enhance ALA metabolism which promotes angiogenesis. Our study provides new insights into mutualistic cellular crosstalk between hair follicles and blood vessels, and identifies novel signaling contributing to the interactions of hair follicles and blood vessels in normal and aged skin.

Keywords: Aging; Angiogenesis; Cell interaction; Hair regeneration; Metabolism; Mutualism.