Three undescribed dihydrostilbene glycosides from leaves of Camellia oleifera Abel. And their anti-inflammatory activity

Heliyon. 2024 Apr 30;10(9):e30507. doi: 10.1016/j.heliyon.2024.e30507. eCollection 2024 May 15.

Abstract

Three previously unidentified dihydrostilbene glycosides, named oleiferaside A (1), oleiferaside B (2), and oleiferaside C (3), were discovered through a phytochemical exploration on Camellia oleifera Abel. leaves. Additionally, nine known secondary metabolites (4-12) were also identified. The undescribed secondary metabolites 1-3 were elucidated as 3,5-dimethoxydihydrostilbene 4'-O-α-l-arabinofuranosyl-(1 → 6)-β-d- glucopyranoside, 3,5-dimethoxydihydrostilbene 4'-O-α-l-arabinopyranosyl-(1 → 6)-β-d- glucopyranoside and 3,5-dimethoxydihydrostilbene 4'-O-β-d-apiofuranosyl-(1 → 6)-β-d- glucopyranoside, respectively. HR-MS and NMR spectroscopy were utilized for determining the structures of the isolates. The natural products were assessed for their anti-inflammatory effect using RAW264.7 macrophage stimulated by LPS. The findings demonstrated that compounds 1-4 exhibited inhibitory activities on NO and PGE2 production without causing cytotoxicity. These observations suggest that these compounds may have potential anti-inflammatory properties.

Keywords: Anti-inflammatory; Camellia oleifera Abel.; Dihydrostilbene glycosides; Oleiferaside A; Oleiferaside B; Oleiferaside C.