Genomic identification and expression analysis of acid invertase (AINV) gene family in Dendrobium officinale Kimura et Migo

Yujia Liu; Boting Liu; Kefa Luo; Baiyin Yu; Xiang Li; Jian Zeng; Jie Chen; Rui Xia; Jing Xu; Yuanlong Liu

doi:10.1186/s12870-024-05102-8

Genomic identification and expression analysis of acid invertase (AINV) gene family in Dendrobium officinale Kimura et Migo

BMC Plant Biol. 2024 May 14;24(1):396. doi: 10.1186/s12870-024-05102-8.

Authors

Yujia Liu^#^{1

2}, Boting Liu^#², Kefa Luo², Baiyin Yu^{3

4}, Xiang Li^{1

2}, Jian Zeng^{1

2}, Jie Chen^{1

2}, Rui Xia^{5

6}, Jing Xu⁷, Yuanlong Liu^{8

9

10}

Affiliations

¹ Guangdong Province Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northerrn Region, Shaoguan University, Shaoguan, Guangdong, 512005, China.
² College of Biology and Agriculture, Shaoguan University, Shaoguan, Guangdong, 512005, China.
³ Guangdong Province Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northerrn Region, Shaoguan University, Shaoguan, Guangdong, 512005, China. 540391135@qq.com.
⁴ College of Biology and Agriculture, Shaoguan University, Shaoguan, Guangdong, 512005, China. 540391135@qq.com.
⁵ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
⁶ College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
⁷ College of Horticulture, South China Agricultural University, Guangzhou, 510642, China. jxu@scau.edu.cn.
⁸ Guangdong Province Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northerrn Region, Shaoguan University, Shaoguan, Guangdong, 512005, China. liuyuanlong@scau.edu.cn.
⁹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China. liuyuanlong@scau.edu.cn.
¹⁰ College of Horticulture, South China Agricultural University, Guangzhou, 510642, China. liuyuanlong@scau.edu.cn.

^# Contributed equally.

Abstract

Background: Dendrobium officinale Kimura et Migo, a renowned traditional Chinese orchid herb esteemed for its significant horticultural and medicinal value, thrives in adverse habitats and contends with various abiotic or biotic stresses. Acid invertases (AINV) are widely considered enzymes involved in regulating sucrose metabolism and have been revealed to participate in plant responses to environmental stress. Although members of AINV gene family have been identified and characterized in multiple plant genomes, detailed information regarding this gene family and its expression patterns remains unknown in D. officinale, despite their significance in polysaccharide biosynthesis.

Results: This study systematically analyzed the D. officinale genome and identified four DoAINV genes, which were classified into two subfamilies based on subcellular prediction and phylogenetic analysis. Comparison of gene structures and conserved motifs in DoAINV genes indicated a high-level conservation during their evolution history. The conserved amino acids and domains of DoAINV proteins were identified as pivotal for their functional roles. Additionally, cis-elements associated with responses to abiotic and biotic stress were found to be the most prevalent motif in all DoAINV genes, indicating their responsiveness to stress. Furthermore, bioinformatics analysis of transcriptome data, validated by quantitative real-time reverse transcription PCR (qRT-PCR), revealed distinct organ-specific expression patterns of DoAINV genes across various tissues and in response to abiotic stress. Examination of soluble sugar content and interaction networks provided insights into stress release and sucrose metabolism.

Conclusions: DoAINV genes are implicated in various activities including growth and development, stress response, and polysaccharide biosynthesis. These findings provide valuable insights into the AINV gene amily of D. officinale and will aid in further elucidating the functions of DoAINV genes.

Keywords: Dendrobium officinale; Abiotic stress; Acid invertase; Expression analysis; Genomic identification; Protein–protein interaction.

MeSH terms

Dendrobium* / enzymology
Dendrobium* / genetics
Gene Expression Profiling
Gene Expression Regulation, Plant*
Genes, Plant
Genome, Plant
Multigene Family*
Phylogeny*
Plant Proteins / genetics
Plant Proteins / metabolism
Stress, Physiological / genetics
beta-Fructofuranosidase* / genetics
beta-Fructofuranosidase* / metabolism