Discovery of Novel p53-MDM2 Inhibitor (RG7388)-Conjugated PlatinumIV Complexes as Potent Antitumor Agents

J Med Chem. 2024 Jun 13;67(11):9645-9661. doi: 10.1021/acs.jmedchem.4c00784. Epub 2024 May 22.

Abstract

While a number of p53-MDM2 inhibitors have progressed into clinical trials for the treatment of cancer, their progression has been hampered by a variety of problems, including acquired drug resistance, dose-dependent toxicity, and limited clinical efficiency. To make more progress, we integrated the advantages of MDM2 inhibitors and platinum drugs to construct novel PtIV-RG7388 (a selective MDM2 inhibitor) complexes. Most complexes, especially 5a and 5b, displayed greatly improved antiproliferative activity against both wild-type and mutated p53 cancer cells. Remarkably, 5a exhibited potent in vivo tumor growth inhibition in the A549 xenograft model (66.5%) without apparent toxicity. It arrested the cell cycle at both the S phase and the G2/M phase and efficiently induced apoptosis via the synergistic effects of RG7388 and cisplatin. Altogether, PtIV-RG7388 complex 5a exhibited excellent in vitro and in vivo antitumor activities, highlighting the therapeutic potential of PtIV-RG7388 complexes as antitumor agents.

MeSH terms

  • Animals
  • Antineoplastic Agents* / chemical synthesis
  • Antineoplastic Agents* / chemistry
  • Antineoplastic Agents* / pharmacology
  • Antineoplastic Agents* / therapeutic use
  • Apoptosis / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Drug Discovery
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Organoplatinum Compounds / chemical synthesis
  • Organoplatinum Compounds / chemistry
  • Organoplatinum Compounds / pharmacology
  • Proto-Oncogene Proteins c-mdm2* / antagonists & inhibitors
  • Proto-Oncogene Proteins c-mdm2* / metabolism
  • Pyrrolidines
  • Structure-Activity Relationship
  • Tumor Suppressor Protein p53* / antagonists & inhibitors
  • Tumor Suppressor Protein p53* / metabolism
  • Xenograft Model Antitumor Assays
  • para-Aminobenzoates

Substances

  • Antineoplastic Agents
  • Proto-Oncogene Proteins c-mdm2
  • Tumor Suppressor Protein p53
  • RG7388
  • MDM2 protein, human
  • Organoplatinum Compounds
  • Pyrrolidines
  • para-Aminobenzoates