Development of silanized bacterial cellulose aerogels for the incorporation of natural oils with healing properties: Copaiba (Copaifera officinalis), bourbon geranium (Pelargonium X ssp.) essential oils and buriti (Mauritia flexuosa) vegetable oil

Int J Biol Macromol. 2024 Jun;269(Pt 2):132266. doi: 10.1016/j.ijbiomac.2024.132266. Epub 2024 May 22.

Abstract

Bacterial cellulose (BC) represents a promising biomaterial, due to its unique and versatile properties. We report, herein, on purposely-designed structural modifications of BC that enhance its application as a wound dressing material. Chemical modification of the functional groups of BC was performed initially to introduce a hydrophobic/oleophilic character to its surface. Specifically, silanization was carried out in an aqueous medium using methyltrimethoxisilane (MTMS) as the silanizing agent, and aerogels were subsequently prepared by freeze-drying. The BC-MTMS aerogel obtained displayed a highly porous (99 %) and lightweight structure with an oil absorption capacity of up to 52 times its dry weight. The XRD pattern indicated that the characteristic crystallographic planes of the native BC were maintained after the silanization process. Thermal analysis showed that the thermal stability of the BC-MTMS aerogel increased, as compared to the pure BC aerogel (pBC). Moreover, the BC-MTMS aerogel was not cytotoxic to fibroblasts and keratinocytes. In the second step of the study, the incorporation of natural oils into the aerogel's matrix was found to endow antimicrobial and/or healing properties to BC-MTMS. Bourbon geranium (Pelargonium X ssp.) essential oil (GEO) was the only oil that exhibited antimicrobial activity against the tested microorganisms, whereas buriti (Mauritia flexuosa) vegetable oil (BVO) was non-cytotoxic to the cells. This study demonstrates that the characteristics of the BC structure can be modified, while preserving its intrinsic features, offering new possibilities for the development of BC-derived materials for specific applications in the biomedical field.

Keywords: Antimicrobial activity; Bacterial cellulose aerogel; Natural oils; Silanization; Wound dressing.

MeSH terms

  • Cellulose* / chemistry
  • Cellulose* / pharmacology
  • Fabaceae / chemistry
  • Fibroblasts / drug effects
  • Gels / chemistry
  • Humans
  • Oils, Volatile* / chemistry
  • Oils, Volatile* / pharmacology
  • Pelargonium / chemistry
  • Plant Oils* / chemistry
  • Plant Oils* / pharmacology
  • Silanes / chemistry
  • Wound Healing / drug effects

Substances

  • Cellulose
  • Oils, Volatile
  • Plant Oils
  • Gels
  • Silanes