Comparison of LIRADS 5 Image Guided Core Biopsy Derived From Formalin Fixed and Frozen Tissue Cores for Radiogenomics and Radioproteomics Analysis in Well, Moderate and Poorly Differentiated Hepatocellular Carcinoma

Margaret Simonian; David S K Lu; Julian Whitelegge; Whitaker Cohn; Preeti Ahuja; William Hsu; Steven S Raman

Comparison of LIRADS 5 Image Guided Core Biopsy Derived From Formalin Fixed and Frozen Tissue Cores for Radiogenomics and Radioproteomics Analysis in Well, Moderate and Poorly Differentiated Hepatocellular Carcinoma

Glob Med Ther. 2024:6:https://www.oatext.com/comparison-of-lirads-5-image-guided-core-biopsy-derived-from-formalin-fixed-and-frozen-tissue-cores-for-radiogenomics-and-radioproteomics-analysis-in-well-moderate-and-poorly-differentiated-hepatocellular-carcinoma.php. Epub 2024 Apr 22.

Authors

Margaret Simonian¹, David S K Lu¹, Julian Whitelegge², Whitaker Cohn², Preeti Ahuja¹, William Hsu¹, Steven S Raman¹

Affiliations

¹ Dept of Radiological Sciences, David Geffen School of Medicine, UCLA, USA.
² Semel Institute for Neuroscience & Human Behavior, Pasarow mass spectrometry lab, David Geffen School of Medicine, UCLA, USA.

PMID: 38818084
PMCID: PMC11138132

Abstract

The aim of this pilot study is to evaluate and compare the quality of the genomics and proteomics data obtained from paired Formalin Fixed Paraffin Embedded (FFPE) and frozen (FF) tissue percutaneous core biopsies of Liver Imaging Reporting and Data System 5 (LIRADS 5) hepatocellular carcinoma (HCC) of varying histological grades. The preliminary data identified differentially expressed proteins and genes in poor, moderate and well differentiated HCC biopsies, with a greater efficacy in fresh frozen samples. The data offered valuable insights into the characteristics and suitability of samples for future studies.

Keywords: Liver HCC; biomarkers; radiogenomics; radioproteomics.

Abstract

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