Efficient purification of somatomedin-C/insulin-like growth factor I using immunoaffinity chromatography

Biochem Biophys Res Commun. 1985 Jan 16;126(1):282-8. doi: 10.1016/0006-291x(85)90603-5.

Abstract

Somatomedin-C/insulin-like growth factor I was purified from human plasma using a monoclonal antibody affinity column. Combining immunoaffinity chromatography with standard protein purification methods resulted in an overall recovery of 18%. The 35 micrograms of somatomedin-C/insulin-like growth factor I purified from 500 ml of plasma appeared as a single band when analyzed by polyacrylamide gel electrophoresis and could be used in radioimmunoassay and receptor binding studies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Chromatography, Affinity / methods
  • Chromatography, Liquid
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Insulin / isolation & purification*
  • Insulin-Like Growth Factor I
  • Isoelectric Focusing
  • Peptides / isolation & purification*
  • Somatomedins / isolation & purification*

Substances

  • Insulin
  • Peptides
  • Somatomedins
  • Insulin-Like Growth Factor I