High variance in quantification of Mycobacterium tuberculosis at low bacterial loads and with differentially detectable mycobacteria

Antimicrob Agents Chemother. 2024 Jul 9;68(7):e0160123. doi: 10.1128/aac.01601-23. Epub 2024 Jun 3.

Abstract

We examined the correlation between three different methods of Mycobacterium tuberculosis quantification: time to positivity (TTP), log10 CFU, and an assay to detect differentially detectable M. tuberculosis (DD Mtb) from three different prospective studies. Participants with DD Mtb have significantly more variation in the CFU/TTP correlation than participants with no DD Mtb (P < 0.001). This may impact the design of early bactericidal activity studies that use TTP as the primary outcome.

Keywords: Mycobacterium tuberculosis; differentially detectable; early bactericidal; quantification.

MeSH terms

  • Adult
  • Bacterial Load* / methods
  • Female
  • Humans
  • Male
  • Mycobacterium tuberculosis* / drug effects
  • Prospective Studies