Soluble levels of 4-1BB (CD137) and OX40 (CD134) are associated with cancer progression in gastric adenocarcinoma

J Surg Oncol. 2024 Sep;130(4):734-740. doi: 10.1002/jso.27726. Epub 2024 Jun 10.

Abstract

Background and objectives: Previous studies have demonstrated that soluble forms of T-cell costimulatory molecules 4-1BB (s4-1BB) and OX40 (sOX40) interact with immune cells and may constitute a mechanism of immune evasion by tumors in various cancers. The role of the soluble forms of 4-1BB and OX40 in GC remains unclear. We aimed to examine the association between serum levels of s4-1BB and sOX40 and tumor progression in patients with GC.

Methods: Between 2017 and 2018, a cross-sectional study was performed with serum samples of 83 GC patients and 20 healthy controls.

Results: Patients with stage IV metastatic gastric cancer had significantly higher levels of soluble OX40 in comparison with stage III patients with lymph nodes metastasis (p = 0.0003) and stages I and II patients (p = 0.005), whereas the opposite was found for soluble 4-1BB levels, with lower levels being found in advanced stage III (p = 0.003) compared with initial stages I/II.

Conclusions: The sOX40 and s4-1BB-mediated T cell interactions may be involved in antitumor immune responses in GC, possibly favoring tumor escape and progression. Serum levels of sOX40 and s4-1BB are associated with staging in GC and may constitute biomarkers for prognosis, as well as potential targets for immunotherapy.

Keywords: gastric cancer; immunotherapy; receptor 4‐1BB; receptor OX40; tumor escape; tumor necrosis factor receptor superfamily.

MeSH terms

  • Adenocarcinoma* / blood
  • Adenocarcinoma* / pathology
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor* / blood
  • Case-Control Studies
  • Cross-Sectional Studies
  • Disease Progression*
  • Female
  • Follow-Up Studies
  • Humans
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Prognosis
  • Receptors, OX40* / blood
  • Receptors, OX40* / metabolism
  • Stomach Neoplasms* / blood
  • Stomach Neoplasms* / pathology
  • Tumor Necrosis Factor Receptor Superfamily, Member 9* / blood
  • Tumor Necrosis Factor Receptor Superfamily, Member 9* / metabolism

Substances

  • Tumor Necrosis Factor Receptor Superfamily, Member 9
  • Receptors, OX40
  • Biomarkers, Tumor
  • TNFRSF4 protein, human
  • TNFRSF9 protein, human