Stable isotopes (15N) facilitate non-invasive labelling of large quantities of macroinvertebrates across different species and feeding types

Julian Enss; Milen Nachev; Maik A Jochmann; Torsten C Schmidt; Christian K Feld

doi:10.1002/ece3.11539

Stable isotopes (¹⁵N) facilitate non-invasive labelling of large quantities of macroinvertebrates across different species and feeding types

Ecol Evol. 2024 Jun 18;14(6):e11539. doi: 10.1002/ece3.11539. eCollection 2024 Jun.

Authors

Julian Enss^{1

2}, Milen Nachev^{1

2}, Maik A Jochmann^{2

3}, Torsten C Schmidt^{2

3}, Christian K Feld^{1

2}

Affiliations

¹ Aquatic Ecology, Faculty of Biology University of Duisburg-Essen Essen Germany.
² Centre for Water and Environmental Research University of Duisburg-Essen Essen Germany.
³ Instrumental Analytical Chemistry, Faculty of Chemistry University of Duisburg-Essen Essen Germany.

Abstract

While macroinvertebrate dispersal operates at the individual level, predictions of their dispersal capabilities often rely on indirect proxies rather than direct measurements. To gain insight into the dispersal of individual specimens, it is crucial to mark (label) and capture individuals. Isotopic enrichment with ¹⁵N is a non-invasive method with the potential of labelling large quantities of macroinvertebrates. While the analysis of ¹⁵N is widely utilised in food web studies, knowledge on the specific utility of isotopic enrichment with ¹⁵N for mass labelling of macroinvertebrate individuals across different taxa and feeding types is limited. Previous studies have focused on single species and feeding types, leaving gaps in our understanding of the broader applicability of this method. Therefore, this study aimed to test and compare isotopic mass enrichment across several macroinvertebrate taxa and feeding types. We released ¹⁵NH₄Cl at five stream reaches in North-Rhine Westphalia, Germany, and successfully enriched 12 distinct macroinvertebrate taxa (Crustacea and Insecta). Significant enrichment was achieved in active and passive filter feeders, grazers, shredders and predators, and predominantly showed positive correlations with the enrichment of the taxa's main food sources phytobenthos and particulate organic matter. Enrichment levels rose rapidly and peaked at distances between 50 m and 300 m downstream of the isotopic inlet; significant enrichment occurred up to 2000 m downstream of the isotopic inlet in all feeding types. Macroinvertebrate density estimates on the stream bottom averaged to a total of approximately 3.4 million labelled individuals of the 12 investigated taxa, thus showing the high potential of isotopic (¹⁵N) enrichment as a non-invasive method applicable for mass labelling across different macroinvertebrate feeding types. Hence, isotopic enrichment can greatly assist the analysis of macroinvertebrate dispersal through mark-and-recapture experiments, as it allows to measure the movement at the level of individual specimens.

Keywords: 15NH4Cl; POM; dispersal measurement; isotopic enrichment; lowland stream; phytobenthos.