Resolvin D1 delivery to lesional macrophages using antioxidative black phosphorus nanosheets for atherosclerosis treatment

Nat Nanotechnol. 2024 Sep;19(9):1386-1398. doi: 10.1038/s41565-024-01687-1. Epub 2024 Jun 19.

Abstract

The buildup of plaques in atherosclerosis leads to cardiovascular events, with chronic unresolved inflammation and overproduction of reactive oxygen species (ROS) being major drivers of plaque progression. Nanotherapeutics that can resolve inflammation and scavenge ROS have the potential to treat atherosclerosis. Here we demonstrate the potential of black phosphorus nanosheets (BPNSs) as a therapeutic agent for the treatment of atherosclerosis. BPNSs can effectively scavenge a broad spectrum of ROS and suppress atherosclerosis-associated pro-inflammatory cytokine production in lesional macrophages. We also demonstrate ROS-responsive, targeted-peptide-modified BPNS-based carriers for the delivery of resolvin D1 (an inflammation-resolving lipid mediator) to lesional macrophages, which further boosts the anti-atherosclerotic efficacy. The targeted nanotherapeutics not only reduce plaque areas but also substantially improve plaque stability in high-fat-diet-fed apolipoprotein E-deficient mice. This study presents a therapeutic strategy against atherosclerosis, and highlights the potential of BPNS-based therapeutics to treat other inflammatory diseases.

MeSH terms

  • Animals
  • Apolipoproteins E / genetics
  • Atherosclerosis* / drug therapy
  • Atherosclerosis* / metabolism
  • Atherosclerosis* / pathology
  • Diet, High-Fat / adverse effects
  • Docosahexaenoic Acids* / administration & dosage
  • Docosahexaenoic Acids* / chemistry
  • Docosahexaenoic Acids* / pharmacology
  • Humans
  • Macrophages* / drug effects
  • Macrophages* / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nanostructures* / chemistry
  • Phosphorus* / chemistry
  • Plaque, Atherosclerotic / drug therapy
  • Plaque, Atherosclerotic / metabolism
  • Plaque, Atherosclerotic / pathology
  • RAW 264.7 Cells
  • Reactive Oxygen Species* / metabolism

Substances

  • Apolipoproteins E
  • Docosahexaenoic Acids
  • Phosphorus
  • Reactive Oxygen Species
  • resolvin D1