7-MEGA™ inhibits adipogenesis in 3T3-L1 adipocytes and suppresses obesity in high-fat-diet-induced obese C57BL/6 mice

Lipids Health Dis. 2024 Jun 22;23(1):192. doi: 10.1186/s12944-024-02175-0.

Abstract

Background: Overweight, often known as obesity, is the abnormal and excessive accumulation of fat that exposes the health of a person at risk by increasing the likelihood that they may experience many chronic conditions. Consequently, obesity has become a global health threat, presenting serious health issues, and attracting a lot of attention in the healthcare profession and the scientific community.

Method: This study aims to explore the anti-adipogenic properties of 7-MEGA™ in an attempt to address obesity, using both in vitro and in vivo research. The effects of 7MEGA™ at three distinct concentrations were investigated in obese mice who were given a high-fat diet (HFD) and 3T3-L1 adipocytes.

Results: 7MEGA™ decreased the total fat mass, overall body weight, and the perirenal and subcutaneous white adipose tissue (PWAT and SWAT) contents in HFD mice. Additionally, 7MEGA™ showed promise in improving the metabolic health of individuals with obesity and regulate the levels of insulin hormone, pro-inflammatory cytokines and adipokines. Furthermore, Peroxisome proliferator-activated receptors (PPAR) α and γ, Uncoupling Protein 1 (UCP-1), Sterol Regulatory Element-Binding Protein 1 (SREBP-1), Fatty Acid-Binding Protein 4 (FABP4), Fatty Acid Synthase (FAS), Acetyl-CoA Carboxylase (ACC), Stearoyl-CoA Desaturase-1 (SCD-1) and CCAAT/Enhancer-Binding Protein (C/EBPα) were among the adipogenic regulators that 7MEGA™ could regulate.

Conclusion: In summary, this study uncovered that 7MEGA™ demonstrates anti-adipogenic and anti-obesity effects, suggesting its potential in combating obesity.

Keywords: Adipocytes; Insulin; Lipid metabolism; Obesity; PPARs; Palmitoleic acid.

MeSH terms

  • 3T3-L1 Cells*
  • Adipocytes* / drug effects
  • Adipocytes* / metabolism
  • Adipogenesis* / drug effects
  • Adipokines / metabolism
  • Adipose Tissue, White / drug effects
  • Adipose Tissue, White / metabolism
  • Animals
  • Anti-Obesity Agents / pharmacology
  • CCAAT-Enhancer-Binding Proteins
  • Diet, High-Fat* / adverse effects
  • Fatty Acid-Binding Proteins / genetics
  • Fatty Acid-Binding Proteins / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL*
  • Mice, Obese
  • Obesity* / metabolism
  • PPAR gamma / genetics
  • PPAR gamma / metabolism
  • Stearoyl-CoA Desaturase / genetics
  • Stearoyl-CoA Desaturase / metabolism
  • Sterol Regulatory Element Binding Protein 1 / genetics
  • Sterol Regulatory Element Binding Protein 1 / metabolism
  • Uncoupling Protein 1 / genetics
  • Uncoupling Protein 1 / metabolism

Substances

  • PPAR gamma
  • Sterol Regulatory Element Binding Protein 1
  • Stearoyl-CoA Desaturase
  • Fatty Acid-Binding Proteins
  • Adipokines
  • Fabp4 protein, mouse
  • CEBPA protein, mouse
  • Anti-Obesity Agents
  • Scd1 protein, mouse
  • Uncoupling Protein 1
  • CCAAT-Enhancer-Binding Proteins