Antimicrobial Composites Based on Methacrylic Acid-Methyl Methacrylate Electrospun Fibers Stabilized with Copper(II)

Molecules. 2024 Jun 14;29(12):2835. doi: 10.3390/molecules29122835.

Abstract

This study presents fibers based on methacrylic acid-methyl methacrylate (Eudragit L100) as Cu(II) adsorbents, resulting in antimicrobial complexes. Eudragit L100, an anionic copolymer synthesized by radical polymerization, was electrospun in dimethylformamide (DMF) and ethanol (EtOH). The electrospinning process was optimized through a 22-factorial design, with independent variables (copolymer concentration and EtOH/DMF volume ratio) and three repetitions at the central point. The smallest average fiber diameter (259 ± 53 nm) was obtained at 14% w/v Eudragit L100 and 80/20 EtOH/DMF volume ratio. The fibers were characterized using scanning electron microscopy (SEM), infrared spectroscopy in attenuated total reflectance mode (FTIR-ATR), and differential scanning calorimetry (DSC). The pseudo-second-order mechanism explained the kinetic adsorption toward Cu(II). The fibers exhibited a maximum adsorption capacity (qe) of 43.70 mg/g. The DSC analysis confirmed the Cu(II) absorption, indicating complexation between metallic ions and copolymer networks. The complexed fibers showed a lower degree of swelling than the non-complexed fibers. The complexed fibers exhibited bacteriostatic activity against Gram-negative (Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria. This study successfully optimized the electrospinning process to produce thin fibers based on Eudragit L100 for potential applications as adsorbents for Cu(II) ions in aqueous media and for controlling bacterial growth.

Keywords: Eudragit L100; bactericidal; electrospinning; optimization.

MeSH terms

  • Adsorption
  • Anti-Infective Agents / chemistry
  • Anti-Infective Agents / pharmacology
  • Calorimetry, Differential Scanning
  • Copper* / chemistry
  • Kinetics
  • Methacrylates / chemistry
  • Microbial Sensitivity Tests
  • Polymethacrylic Acids* / chemistry
  • Spectroscopy, Fourier Transform Infrared
  • Staphylococcus aureus / drug effects

Substances

  • Copper
  • Polymethacrylic Acids
  • Anti-Infective Agents
  • methylmethacrylate-methacrylic acid copolymer
  • Methacrylates

Grants and funding

This research was funded by “National Council for Scientific and Technological Development–CNPq” for financial support (grant 305579/2023-1). “This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Finance Code 001”.