Optical coherence tomography of the macular ganglion cell layer in children with neurofibromatosis type 1 is a useful tool in the assessment for optic pathway gliomas

PLoS One. 2024 Jul 11;19(7):e0305548. doi: 10.1371/journal.pone.0305548. eCollection 2024.

Abstract

Background: Optic pathway glioma (OPG) is a feared complication to neurofibromatosis type 1 (NF1) since it can cause visual impairment in young children. The main goal of screening is to detect symptomatic OPGs that require treatment. Optical coherence tomography (OCT) has been suggested as a tool for detection of neuro-retinal damage.

Aims: To investigate whether the ganglion cell layer assessed by OCT is a reliable measure to identify and detect relapses of symptomatic OPGs in children with NF1.

Methods: Children (3-6 years) with NF1, with and without known OPG and children with sporadic OPG (S-OPG) resident in the Stockholm area, were invited and followed in a prospective study during a three-year period. Brain magnetic resonance tomography (MRI) had been performed in children with symptoms of OPG. Outcome measures were VA in logMAR, visual field index (VFI), average thicknesses of the ganglion cell-inner plexiform layer (GC-IPL), and peripapillary retinal nerve fiber layer (pRNFL).

Results: There were 25 children with MRI-verified OPG and 52 with NF1 without symptomatic OPG. Eyes from NF1 patients without symptoms of OPG showed significantly better results in all four analyzed parameters compared to eyes with NF1-associated OPG. Mean GC-IPL measurements seemed stable and reliable, significantly correlated to pRNFL (correlation coefficient (r) = 0.662, confidence interval (CI) = .507 to .773 p<0.001), VA (r = -0.661, CI = -7.45 to -.551, p<0.001) and VFI (r = 0.644, CI = .452 to .774, p<0.001). GC-IPL measurements were easy to obtain and acquired at considerably younger age than pRNFL (5.6±1.5 vs 6.8±1.3; p<0.001).

Conclusions: The mean GC-IPL thickness could distinguish well between eyes with OPG and eyes without symptomatic OPG in children with NF1. As thinning of GC-IPL assessed with OCT could indicate underlying OPG, it should be included in the screening protocol of children with questionable VA measurements and in particular in children with NF1.

MeSH terms

  • Child
  • Child, Preschool
  • Female
  • Humans
  • Magnetic Resonance Imaging / methods
  • Male
  • Neurofibromatosis 1* / diagnostic imaging
  • Optic Nerve Glioma* / diagnostic imaging
  • Prospective Studies
  • Retinal Ganglion Cells* / pathology
  • Tomography, Optical Coherence* / methods
  • Visual Acuity

Grants and funding

This research was funded by the Swedish Childhood Cancer Fund (KF2016-0015, KF2016- 0020), the Stockholm County Council and Karolinska Institute (grant numbers ALF-20160227, FoUI 947257, 949327, 951235, 952920), the Sigvard & Marianne Bernadotte Research Foundation for Children Eye Care.