Oral administration of GnRH via a cricket vehicle stimulates spermiation in tiger salamanders (Ambystoma tigrinum)

PLoS One. 2024 Jul 25;19(7):e0289995. doi: 10.1371/journal.pone.0289995. eCollection 2024.

Abstract

More than 50% of caudates are threatened with extinction and are in need of ex-situ breeding programs to support conservation efforts and species recovery. Unfortunately, many salamander populations under human care can experience reproductive failure, primarily due to missing environmental cues necessary for breeding. Assisted reproductive technologies (ARTs) are a useful suite of techniques for overcoming or bypassing these missing environmental cues to promote breeding. Exogenous hormones are used to stimulate natural breeding behaviors or gamete expression for in-vitro fertilization or biobanking and are typically administered intramuscularly in caudates. While effective, intramuscular injection is risky to perform in smaller-bodied animals, resulting in health and welfare risks. This research investigated the spermiation response to hormone administration through a non-invasive oral bioencapsulation route using the tiger salamander (Ambystoma tigrinum) as a model species. Male salamanders were randomly rotated six weeks apart through four treatments (n = 11 males/treatment) in which animals received a resolving dose of gonadotropin-releasing hormone (GnRH) as follows: (1) Prime-Only (0.0 μg/g); (2) Low (0.25 μg/g); (3) Medium (1.0 μg/g); and (4) High (2.0 μg/g). All males were given a GnRH priming dose (0.25 μg/g) 24 hours prior to the resolving dose. Exogenous hormone was delivered inside of a cricket (Gryllodes sigillatus) that was presented as a food item by tweezers. Sperm samples were collected at 1, 3, 6, 9, 12, and 24 hours after the resolving dose and analyzed for quantity and quality. For all treatments, sperm concentration was produced in an episodic pattern over time. The Prime-Only treatment had a lower (p < 0.05) percent of sperm exhibiting normal morphology compared to treatments utilizing a resolving dose of GnRH. Overall, oral administration of GnRH is a feasible route of inducing spermiation in salamanders, yielding sperm of sufficient quantity and quality for in-vitro fertilization and biobanking efforts.

MeSH terms

  • Administration, Oral
  • Ambystoma*
  • Animals
  • Gonadotropin-Releasing Hormone* / administration & dosage
  • Gryllidae
  • Male
  • Spermatogenesis / drug effects
  • Spermatozoa / drug effects

Substances

  • Gonadotropin-Releasing Hormone

Grants and funding

This work was supported by the Institute of Museum and Library Services (Grant #MG-30-17-0052-17 awarded to CKK and AJK; https://www.imls.gov/), Mississippi State Forest and Wildlife Research Center, Mississippi Agricultural and Forestry Experiment Station (#W3173 awarded to CKK; https://www.mafes.msstate.edu), and U.S. Department of Agriculture, Agricultural Research Service (#6066-31000-015-00D awarded to CKK; https://www.ars.usda.gov). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.