Improvements in hematologic markers and decreases in fatigue with pegcetacoplan for patients with paroxysmal nocturnal hemoglobinuria and mild or moderate anemia (hemoglobin ≥10 g/dL) who had received eculizumab or were naive to complement inhibitors

PLoS One. 2024 Jul 29;19(7):e0306407. doi: 10.1371/journal.pone.0306407. eCollection 2024.

Abstract

Background: Although complement component 5 inhibitors (C5is) eculizumab and ravulizumab improve paroxysmal nocturnal hemoglobinuria (PNH) outcomes, patients may experience persistent anemia. This post hoc analysis investigated whether the complement component 3-targeted therapy pegcetacoplan also improved hematologic outcomes and reduced fatigue in patients with PNH and mild/moderate anemia.

Methods: Patients with PNH and hemoglobin ≥10.0 g/dL at baseline of PADDOCK (N = 6), PRINCE (N = 8), and PEGASUS (N = 11) were included. Before receiving pegcetacoplan, PADDOCK and PRINCE patients were C5i-naive; PEGASUS patients had hemoglobin <10.5 g/dL despite stably dosed eculizumab. Hemoglobin concentrations, percentages of patients with concentrations ≥12 g/dL, and sex-specific normalization were assessed at baseline and after 16 weeks of pegcetacoplan, as were absolute reticulocyte counts (ARCs) and normalization and fatigue scores and normalization.

Results: From baseline to week 16, mean (SD) hemoglobin concentrations increased in C5i-naive patients (PADDOCK: 10.5 [0.4] to 12.7 [1.1] g/dL; PRINCE: 11.3 [1.0] to 14.0 [1.3] g/dL) and those with suboptimal eculizumab responses (PEGASUS: 10.2 [0.2] to 12.8 [2.6] g/dL). Percentage of patients with hemoglobin ≥12 g/dL increased (PADDOCK: 0 to 60.0% [3 of 5 patients]; PRINCE: 25.0% [2 of 8] to 87.5% [7 of 8]; PEGASUS: 0 to 72.7% [8 of 11]). Sex-specific hemoglobin normalization at week 16 occurred in 40.0% (2 of 5) (PADDOCK), 62.5% (5 of 8) (PRINCE), and 63.6% (7 of 11) (PEGASUS). In all studies, mean ARCs decreased from above normal to normal and ARC normalization increased. Mean Functional Assessment of Chronic Illness Therapy-Fatigue scores improved from below to above or near normal. Two patients had serious adverse events (PEGASUS: post-surgery sepsis, breakthrough hemolysis); breakthrough hemolysis resolved without study discontinuation.

Conclusion: Patients with PNH and mild/moderate anemia who were C5i-naive or who had suboptimal hemoglobin concentrations despite eculizumab treatment had improved hematologic outcomes and reduced fatigue after initiating or switching to pegcetacoplan.

Trial registration: Trial registration numbers: PADDOCK (NCT02588833), PRINCE (NCT04085601; EudraCT, 2018-004220-11), PEGASUS (NCT03500549).

Publication types

  • Clinical Trial, Phase I
  • Clinical Trial, Phase III
  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Aged
  • Anemia* / blood
  • Anemia* / drug therapy
  • Anemia* / etiology
  • Antibodies, Monoclonal, Humanized* / therapeutic use
  • Biomarkers / blood
  • Complement C3 / metabolism
  • Complement Inactivating Agents / therapeutic use
  • Fatigue* / blood
  • Fatigue* / drug therapy
  • Fatigue* / etiology
  • Female
  • Hemoglobins* / analysis
  • Hemoglobins* / metabolism
  • Hemoglobinuria, Paroxysmal* / blood
  • Hemoglobinuria, Paroxysmal* / drug therapy
  • Humans
  • Male
  • Middle Aged
  • Treatment Outcome

Substances

  • Antibodies, Monoclonal, Humanized
  • Biomarkers
  • Complement C3
  • Complement Inactivating Agents
  • eculizumab
  • Hemoglobins

Associated data

  • ClinicalTrials.gov/NCT03500549
  • ClinicalTrials.gov/NCT04085601
  • ClinicalTrials.gov/NCT02588833

Grants and funding

This study was sponsored by Apellis Pharmaceuticals, Inc. (https://apellis.com/) and Swedish Orphan Biovitrum AB (https://www.sobi.com/en). The sponsors also contributed to data interpretation and manuscript development. The funder provided support in the form of salaries for authors Mohammed Al-Adhami (employed by Apellis at the time of the study) and Emmelie Persson (employee of Swedish Orphan Biovitrum AB). The specific roles of these authors are articulated in the ‘author contributions’ section. Writing assistance was funded by Apellis Pharmaceuticals, Inc. and Swedish Orphan Biovitrum AB. There was no additional external funding received for this study.