Association of Oral Microbiome Diversity and All-Cause Mortality in the General US Population and in Individuals With Chronic Diseases: A Prospective Cohort Study

J Clin Periodontol. 2024 Nov;51(11):1490-1501. doi: 10.1111/jcpe.14056. Epub 2024 Aug 16.

Abstract

Aim: To investigate whether oral microbiome diversity is associated with all-cause mortality in the general US population and in individuals with chronic diseases.

Materials and methods: We included 8224 individuals with oral microbiome diversity data from the National Health and Nutrition Examination Survey (2009-2012), representing 164,000,205 US adults, using a survey-weighted analysis method. Cox regression analyses were performed to identify the association between oral microbiome diversity and all-cause mortality.

Results: During a survey-weighted mean follow-up period of 8.86 years, 429 all-cause deaths (survey-weighted number: 7,124,920) occurred in 8224 participants. Cox regression analysis revealed that higher oral microbiome diversity was significantly associated with a lower all-cause mortality risk. Significant differences in all-cause mortality risk were observed among the different clusters based on oral microbiome β-diversity (log-rank p < 0.001). Subgroup analyses revealed that the oral microbiome diversity was independently associated with all-cause mortality in individuals with diabetes mellitus and hypertension. A multivariate logistic regression model showed that current smoking and antibiotic use were significantly associated with lower oral microbiome α diversity.

Conclusions: Higher oral microbiome diversity was significantly associated with a lower all-cause mortality risk in the general US population and in individuals with diabetes mellitus and hypertension.

Keywords: extraoral chronic diseases; general US population; lifestyles intervention; long‐term prognosis; oral microbiome diversity.

MeSH terms

  • Adult
  • Aged
  • Cause of Death
  • Chronic Disease
  • Female
  • Humans
  • Male
  • Microbiota*
  • Middle Aged
  • Mouth / microbiology
  • Nutrition Surveys*
  • Prospective Studies
  • United States / epidemiology