Enhanced osteogenesis and antibacterial activity of dual-functional PEEK implants via biomimetic polydopamine modification with chondroitin sulfate and levofloxacin

J Biomater Sci Polym Ed. 2024 Dec;35(18):2790-2806. doi: 10.1080/09205063.2024.2390745. Epub 2024 Aug 18.

Abstract

Polyetheretherketone (PEEK) implants have emerged as a clinically favored alternative to titanium alloy implants for cranial bone substitutes due to their excellent mechanical properties and biocompatibility. However, the biological inertness of PEEK has hindered its clinical application. To address this issue, we developed a dual-functional surface modification method aimed at enhancing both osteogenesis and antibacterial activity, which was achieved through the sustained release of chondroitin sulfate (CS) and levofloxacin (LVFX) from a biomimetic polydopamine (PDA) coating on the PEEK surface. CS was introduced to promote cell adhesion and osteogenic differentiation. Meanwhile, incorporation of antibiotic LVFX was essential to prevent infections, which are a critical concern in bone defect repairing. To our delight, experiment results demonstrated that the SPKD/CS-LVFX specimen exhibited enhanced hydrophilicity and sustained drug release profiles. Furthermore, in vitro experiments showed that cell growth and adhesion, cell viability, and osteogenic differentiation of mouse calvaria-derived osteoblast precursor (MC3T3-E1) cells were significantly improved on the SPKD/CS-LVFX coating. Antibacterial assays also confirmed that the SPKD/CS-LVFX specimen effectively inhibited the growth of Escherichia coli and Staphylococcus aureus, attributable to the antibiotic LVFX released from the PDA coating. To sum up, this dual-functional PEEK implant showed a promising potential for clinical application in bone defects repairing, providing excellent osteogenic and antibacterial properties through a synergistic approach.

Keywords: Biomimetic; PEEK; antibacterial property; chondroitin sulfate; levofloxacin; osteogenesis.

MeSH terms

  • Animals
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Benzophenones* / chemistry
  • Biomimetic Materials / chemistry
  • Biomimetic Materials / pharmacology
  • Cell Adhesion / drug effects
  • Cell Differentiation / drug effects
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Chondroitin Sulfates* / chemistry
  • Chondroitin Sulfates* / pharmacology
  • Coated Materials, Biocompatible / chemistry
  • Coated Materials, Biocompatible / pharmacology
  • Drug Liberation
  • Escherichia coli / drug effects
  • Indoles* / chemistry
  • Indoles* / pharmacology
  • Ketones* / chemistry
  • Ketones* / pharmacology
  • Levofloxacin* / administration & dosage
  • Levofloxacin* / chemistry
  • Levofloxacin* / pharmacology
  • Mice
  • Osteoblasts / cytology
  • Osteoblasts / drug effects
  • Osteogenesis* / drug effects
  • Polyethylene Glycols* / chemistry
  • Polymers* / chemistry
  • Prostheses and Implants
  • Staphylococcus aureus* / drug effects
  • Surface Properties

Substances

  • Indoles
  • polydopamine
  • Polymers
  • Anti-Bacterial Agents
  • Chondroitin Sulfates
  • Benzophenones
  • polyetheretherketone
  • Ketones
  • Levofloxacin
  • Polyethylene Glycols
  • Coated Materials, Biocompatible