Differentiating Ischemic Hepatitis from Acetaminophen Overdose in Acute Liver Failure: Role of Acetaminophen Adducts-Ischemic Hepatitis vs Acetaminophen Overdose

Dig Dis Sci. 2024 Oct;69(10):3952-3961. doi: 10.1007/s10620-024-08602-7. Epub 2024 Sep 2.

Abstract

Background and aims: Acetaminophen (APAP) hepatotoxicity and ischemic hepatic injury (IH) demonstrate remarkably similar biochemical patterns. Deciding between these two etiologies in the setting of acute liver failure (ALF) can be challenging. We reviewed all cases in the Acute Liver Failure Study Group (ALFSG) registry where these diagnoses were considered, to determine reasons for, and frequency of, difficulties making these diagnoses. We hypothesized that the newly developed APAP-CYS adduct assay could help in discerning the correct diagnosis.

Methods: Among 3364 patients with ALF or acute liver injury (ALI: INR ≥ 2.0 but without encephalopathy) between 1998 and 2019, 1952 (58%) received a final diagnosis of either APAP (1681) or IH (271). We utilized a review committee of senior hepatologists as well as the APAP-CYS assay (where sera were available), measuring the presence of toxic by-products of APAP injury to optimize adjudication.

Results: With these methods, a total of 575 adduct positive APAP cases included 488 recognized APAP, as well as an additional 87 patients previously diagnosed as other etiologies. Nine cases initially attributed to IH were deemed combination APAP-IH injuries. Conversely, 215 of the 280 IH subjects tested for adducts disclosed 173 confirmed as IH with adduct testing below the toxicity threshold, while 9 cases were revised from APAP to the IH-APAP combination phenotype, where both hypotension and APAP likely played a role.

Conclusions: Discerning APAP from IH can be difficult-in rare cases, combined injury is observed (18/1952). APAP-CYS testing resulted in revising the diagnosis in 14.6% of cases.

Keywords: Acetaminophen overdose; Cardiac dysfunction; Hepatic necrosis; Hypoxia; Shock liver.

MeSH terms

  • Acetaminophen* / analogs & derivatives
  • Acetaminophen* / poisoning
  • Adult
  • Analgesics, Non-Narcotic / poisoning
  • Chemical and Drug Induced Liver Injury* / blood
  • Chemical and Drug Induced Liver Injury* / diagnosis
  • Chemical and Drug Induced Liver Injury* / etiology
  • Cysteine / analogs & derivatives
  • Cysteine / blood
  • Diagnosis, Differential
  • Drug Overdose* / complications
  • Drug Overdose* / diagnosis
  • Female
  • Humans
  • Ischemia / diagnosis
  • Liver
  • Liver Failure, Acute* / blood
  • Liver Failure, Acute* / chemically induced
  • Liver Failure, Acute* / diagnosis
  • Male
  • Middle Aged
  • Registries
  • Retrospective Studies

Substances

  • Acetaminophen
  • Analgesics, Non-Narcotic
  • Cysteine
  • acetaminophen cysteine