Generation of induced pluripotent stem cells from ADHD patients who do or do not respond to Methylphenidate treatment

Cristine Marie Yde Ohki; Natalie Monet Walter; Michelle Rickli; Cedric Iseli; Anna Maria Werling; Christian Döring; Belén Rubio; Per Hoffmann; Stefan Herms; Susanne Walitza; Edna Grünblatt

doi:10.1016/j.scr.2024.103546

Generation of induced pluripotent stem cells from ADHD patients who do or do not respond to Methylphenidate treatment

Stem Cell Res. 2024 Sep 1:81:103546. doi: 10.1016/j.scr.2024.103546. Online ahead of print.

Affiliations

¹ Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland.
² Human Genomics Research Group, Department of Biomedicine, University of Basel, 4031 Basel, Switzerland; Institute of Human Genetics, University of Bonn, School of Medicine & University Hospital Bonn, 53105 Bonn, Germany.
³ Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.
⁴ Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland. Electronic address: edna.gruenblatt@kjpd.uzh.ch.

PMID: 39241453
DOI: 10.1016/j.scr.2024.103546

Abstract

As a neurodevelopmental multifactorial disorder whose prevalence has been increasing worldwide, attention-deficit hyperactivity disorder (ADHD) is considered a public health concern. Methylphenidate (MPH) is the drug of choice for ADHD; however, not all patients respond fully to this treatment. Therefore, exploring the underlying molecular mechanisms involved in ADHD and potential novel therapeutic targets is crucial. Here, we generated induced pluripotent stem cells (iPSCs) from Peripheral Blood Mononuclear Cells (PBMCs) retrieved from four ADHD patients (two MPH responders and two non-responders) using Sendai virus. These lines might be helpful for the in vitro investigation of ADHD pathophysiology in a patient-specific manner.