Elevated atmospheric CO2 alters the multi-element stoichiometry of pollen-bearing oak flowers, with possible negative effects on bees

Abstract

Increasing atmospheric CO₂ levels change the elemental composition in plants, altering their nutritional quality and affecting consumers and ecosystems. Ecological stoichiometry provides a framework for investigating how CO₂-driven nutrient dilution in pollen affects bees by linking changes in pollen chemical element proportions to the nutritional needs of bees. We investigated the consequences of five years of Free Air CO₂ Enrichment (FACE) in a mature oak-dominated temperate forest on the elemental composition of English oak (Quercus robur) pollen. We measured the concentrations and proportions of 12 elements (C, N, P, S, K, Na, Ca, Mg, Cu, Zn, Fe, and Mn) in Q. robur pollen-bearing flowers collected from the Birmingham Institute for Forest Research (BIFoR) FACE facility. An elevated CO₂ (eCO₂) level of 150 ppm above ambient significantly reduced the S, K, and Fe levels and altered the multi-element ratio, with different elements behaving differently. This shift in pollen multi-element composition may have subsequent cascading effects on higher trophic levels. To assess the impact on bees, we calculated the stoichiometric mismatch (a measure of the discrepancy between consumer needs and food quality) for two bee species, Osmia bicornis (red mason bee) and Apis mellifera (honey bee), that consume oak pollen in nature. We observed stoichiometric mismatches for P and S, in pollen under eCO₂, which could negatively affect bees. We highlight the need for a comprehensive understanding of the changes in pollen multi-element stoichiometry under eCO₂, which leads to nutrient limitations under climate change with consequences for bees.

Keywords: Quercus robur; Climate change; FACE; Nutrition; Pollinator; Temperate forest.