Intermittent Fasting-Induced Orm2 Promotes Adipose Browning via the GP130/IL23R-p38 Cascade

Adv Sci (Weinh). 2024 Nov;11(42):e2407789. doi: 10.1002/advs.202407789. Epub 2024 Sep 9.

Abstract

Intermittent fasting (IF) plays a critical role in mitigating obesity, yet the precise biological mechanisms require further elucidation. Here Orosomucoid 2 (Orm2) is identified as an IF-induced hepatokine that stimulates adipose browning. IF induced Orm2 expression and secretion from the liver through peroxisome proliferator-activated receptor alpha (PPARα). In adipose tissue, Orm2 bound to glycoprotein 130/interleukin 23 receptor (GP130/IL23R) and promoted adipose browning through the activation of p38 mitogen-activated protein kinases (p38-MAPK). In obese mice, Orm2 led to a significant induction of adipose tissue browning and subsequent weight loss, an effect that is not replicated by a mutant variant of Orm2 deficient in GP130/IL23R binding capability. Crucially, genetic association studies in humans identified an obesity-associated Orm2 variant (D178E), which shows decreased GP130/IL23R binding and impaired browning capacity in mice. Overall, the research identifies Orm2 as a promising therapeutic target for obesity, mediating adipose browning through the GP130/IL23R-p38 signalling pathway.

Keywords: GP130/IL23R; Obesity; Orm2; adipose tissue browning; intermittent fasting.

MeSH terms

  • Adipose Tissue, Brown / metabolism
  • Animals
  • Cytokine Receptor gp130 / genetics
  • Cytokine Receptor gp130 / metabolism
  • Disease Models, Animal
  • Fasting* / metabolism
  • Female
  • Humans
  • Intermittent Fasting
  • Male
  • Mice
  • Mice, Inbred C57BL*
  • Mice, Obese
  • Obesity* / genetics
  • Obesity* / metabolism
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin / metabolism
  • Signal Transduction / genetics
  • p38 Mitogen-Activated Protein Kinases* / genetics
  • p38 Mitogen-Activated Protein Kinases* / metabolism

Substances

  • p38 Mitogen-Activated Protein Kinases
  • Cytokine Receptor gp130
  • Receptors, Interleukin