Zuogui Pill Ameliorates Glucocorticoid-Induced Osteoporosis through ZNF702P-Based ceRNA Network: Bioinformatics Analysis and Experimental Validation

Peng Zhang; Honglin Chen; Qi Shang; Guifeng Chen; Jiahui He; Gengyang Shen; Xiang Yu; Zhida Zhang; Wenhua Zhao; Guangye Zhu; Jinglin Huang; De Liang; Jingjing Tang; Jianchao Cui; Zhixiang Liu; Xiaobing Jiang; Hui Ren

doi:10.1155/2022/8020182

Zuogui Pill Ameliorates Glucocorticoid-Induced Osteoporosis through ZNF702P-Based ceRNA Network: Bioinformatics Analysis and Experimental Validation

Evid Based Complement Alternat Med. 2022 Aug 29:2022:8020182. doi: 10.1155/2022/8020182. eCollection 2022.

Authors

Peng Zhang^{1

2

3}, Honglin Chen^{1

2

3}, Qi Shang^{1

2

3}, Guifeng Chen^{1

2

3}, Jiahui He^{1

2

3}, Gengyang Shen^{2

3}, Xiang Yu^{2

3}, Zhida Zhang^{2

3}, Wenhua Zhao^{1

2

3}, Guangye Zhu¹, Jinglin Huang¹, De Liang^{2

3}, Jingjing Tang^{2

3}, Jianchao Cui^{2

3}, Zhixiang Liu⁴, Xiaobing Jiang^{2

3}, Hui Ren^{2

3}

Affiliations

¹ The First Clinical School, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
² The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
³ Lingnan Medical Research Center of Guangzhou Univercity of Chinese Medicine, Guangzhou 510405, China.
⁴ Affiliated Huadu Hospital, Southern Medical University, Guangzhou 510800, China.

Abstract

Glucocorticoid-induced osteoporosis (GIOP) is a musculoskeletal disease with increased fracture risk caused by long-term application of glucocorticoid, but there exist few effective interventions. Zuogui Pill (ZGP) has achieved clinical improvement for GIOP as an ancient classical formula, but its molecular mechanisms remain unclear due to scanty relevant studies. This study aimed to excavate the effective compounds and underlying mechanism of ZGP in treating GIOP and construct relative ceRNA network by using integrated analysis of bioinformatics analysis and experimental validation. Results show that ZNF702P is significantly upregulated in GIOP than normal cases based on gene chip sequencing analysis. Totally, 102 ingredients and 535 targets of ZGP as well as 480 GIOP-related targets were selected, including 122 common targets and 8 intersection targets with the predicted mRNAs. The ceRNA network contains one lncRNA (ZNF702P), 6 miRNAs, and 8 mRNAs. Four hub targets including JUN, CCND1, MAPK1, and MAPK14 were identified in the PPI network. Six ceRNA interaction axes including ZNF702P-hsa-miR-429-JUN, ZNF702P-hsa-miR-17-5p/hsa-miR-20b-5p-CCND1, ZNF702P-hsa-miR-17-5p/hsa-miR-20b-5p-MAPK1, and ZNF702P-hsa-miR-24-3p-MAPK14 were obtained. By means of molecular docking, we found that all the hub targets could be effectively combined with related ingredients. GO enrichment analysis showed 649 biological processes, involving response to estrogen, response to steroid hormone, inflammatory response, macrophage activation, and osteoclast differentiation, and KEGG analysis revealed 102 entries with 36 relative signaling pathways, which mainly contained IL-17 signaling pathway, T cell receptor signaling pathway, FoxO signaling pathway, the PD-L1 expression and PD-1 checkpoint pathway, MAPK signaling pathway, TNF signaling pathway, Estrogen signaling pathway, and Wnt signaling pathway. Our experiments confirmed that ZNF702P exhibited gradually increasing expression levels during osteoclast differentiation of human peripheral blood monocytes (HPBMs) induced by RANKL, while ZGP could inhibit osteoclast differentiation of HPBMs induced by RANKL in a concentration-dependent manner. Therefore, by regulating inflammatory response, osteoclast differentiation, and hormone metabolism, ZGP may treat GIOP by regulating hub target genes, such as JUN, CCND1, MAPK1, and MAPK14, and acting on numerous key pathways, which involve the ZNF702P-based ceRNA network.