Electrochemically grafted gallic acid-chitosan: a metal-free in situ redox platform for regiospecific oligopeptide-viral spike protein interaction

Chem Commun (Camb). 2024 Oct 22;60(85):12405-12408. doi: 10.1039/d4cc04954c.

Abstract

Electrochemical grafting of gallic acid with chitosan (EgGC) voltammetrically deposited on a multitude of substrates exhibiting reversible oxidoreduction suitable for sensor construction is reported. A bioreceptor customized from the fragment antigen binding region of SARS-CoV-2 neutralizing antibodies immobilized on an EgGC matrix supported the selective/specific electrochemical signal transduction with respect to different viral loads (femtogram level) of SARS-CoV-2.

MeSH terms

  • Antibodies, Neutralizing / chemistry
  • Antibodies, Neutralizing / immunology
  • Biosensing Techniques
  • COVID-19 / virology
  • Chitosan* / chemistry
  • Electrochemical Techniques*
  • Gallic Acid* / chemistry
  • Humans
  • Oligopeptides / chemistry
  • Oxidation-Reduction*
  • SARS-CoV-2*
  • Spike Glycoprotein, Coronavirus* / chemistry
  • Spike Glycoprotein, Coronavirus* / metabolism

Substances

  • Gallic Acid
  • Spike Glycoprotein, Coronavirus
  • Chitosan
  • spike protein, SARS-CoV-2
  • Oligopeptides
  • Antibodies, Neutralizing