Unveiling STRs instability in a colorectal cancer FFPE sample: a case report

Giulia Soldati; Chiara Saccardo; Dario Raniero; Domenico De Leo; Stefania Turrina

doi:10.1007/s00414-024-03341-w

Unveiling STRs instability in a colorectal cancer FFPE sample: a case report

Int J Legal Med. 2024 Oct 8. doi: 10.1007/s00414-024-03341-w. Online ahead of print.

Authors

Giulia Soldati¹, Chiara Saccardo², Dario Raniero², Domenico De Leo², Stefania Turrina²

Affiliations

¹ Department of Diagnostics and Public Health, Section of Forensic Medicine, Forensic Genetics Lab, University of Verona, Verona, Italy. giulia.soldati_02@univr.it.
² Department of Diagnostics and Public Health, Section of Forensic Medicine, Forensic Genetics Lab, University of Verona, Verona, Italy.

PMID: 39377931
DOI: 10.1007/s00414-024-03341-w

Abstract

In forensic genetics, sometimes formalin-fixed paraffin-embedded (FFPE) biopsy material taken during life is the only biological sample available for individual identification or paternity testing. In most cases, this biological tissue is characterized by the presence of tumor cells characterized by instability and loss of heterozygosity of microsatellites (MSI/LOH) compared to the DNA present in cells of normal tissue.In this case report, two FFPE samples from the same male subject were available for genetic investigation: one sample with colorectal cancer tissue and the other with normal tissue (no cancerous histopathological features). The comparison of the genetic profiles obtained from DNA extracted from the two tissues showed in the tumor tissue the presence of three genomic instability phenomena affecting FGA, CSF1P0, D21S2055 loci, located on three distinct autosomal chromosomes, and one duplication phenomenon affecting the DYS438. Therefore, due to the MSI/LOH phenomena, the genetic profile acquired from the tumor tissue was distorted and thus generated a fictitious genetic profile, not corresponding to the subject's real one (normal tissue free of tumor cells).

Keywords: Cancerous tissues; Case report; Forensic genetics profiles; Formalin fixed paraffin embedded tissues; Instability of microsatellites; Loss of heterozygosity.