C/EBPδ deficiency delays infection-induced preterm birth

BMC Med. 2024 Oct 8;22(1):432. doi: 10.1186/s12916-024-03650-2.

Abstract

Background: Parturition is an inflammation process. Exaggerated inflammatory reactions in infection lead to preterm birth. Although nuclear factor kappa B (NF-κB) has been recognized as a classical transcription factor mediating inflammatory reactions, those mediated by NF-κB per se are relatively short-lived. Therefore, there may be other transcription factors involved to sustain NF-κB-initiated inflammatory reactions in gestational tissues in infection-induced preterm birth.

Methods: Cebpd-deficient mice were generated to investigate the role of CCAAT enhancer-binding protein δ (C/EBPδ) in lipopolysaccharide (LPS)-induced preterm birth, and the contribution of fetal and maternal C/EBPδ was further dissected by transferring Cebpd-/- or WT embryos to Cebpd-/- or WT dams. The effects of C/EBPδ pertinent to parturition were investigated in mouse and human myometrial and amnion cells. The interplay between C/EBPδ and NF-κB was examined in cultured human amnion fibroblasts.

Results: The mouse study showed that LPS-induced preterm birth was delayed by Cebpd deficiency in either the fetus or the dam, with further delay being observed in conceptions where both the dam and the fetus were deficient in Cebpd. Mouse and human studies showed that the abundance of C/EBPδ was significantly increased in the myometrium and fetal membranes in infection-induced preterm birth. Furthermore, C/EBPδ participated in LPS-induced upregulation of pro-inflammatory cytokines as well as genes pertinent to myometrial contractility and fetal membrane activation in the myometrium and amnion respectively. A mechanistic study in human amnion fibroblasts showed that C/EBPδ, upon induction by NF-κB, could serve as a supplementary transcription factor to NF-κB to sustain the expression of genes pertinent to parturition.

Conclusions: C/EBPδ is a transcription factor to sustain the expression of gene initiated by NF-κB in the myometrium and fetal membranes in infection-induced preterm birth. Targeting C/EBPδ may be of therapeutic value in the treatment of infection-induced preterm birth.

Keywords: C/EBPδ; Fetal membranes; Inflammation; Myometrium; NF-κB; Parturition; Preterm birth.

MeSH terms

  • Animals
  • CCAAT-Enhancer-Binding Protein-delta* / genetics
  • CCAAT-Enhancer-Binding Protein-delta* / metabolism
  • Cells, Cultured
  • Female
  • Fibroblasts / metabolism
  • Humans
  • Lipopolysaccharides*
  • Mice
  • Mice, Knockout
  • NF-kappa B* / metabolism
  • Pregnancy
  • Premature Birth*

Substances

  • CCAAT-Enhancer-Binding Protein-delta
  • NF-kappa B
  • Lipopolysaccharides
  • Cebpd protein, mouse