Mitigating sTNF/TNFR1 activation on VGluT2 + spinal cord interneurons improves immune function after mid-thoracic spinal cord injury

Brain Behav Immun. 2025 Jan:123:633-643. doi: 10.1016/j.bbi.2024.10.021. Epub 2024 Oct 15.

Abstract

Spinal cord injury (SCI) is a devastating condition with 250,000 to 500,000 new cases globally each year. Respiratory infections, e.g., pneumonia and influenza are the leading cause of death after SCI. Unfortunately, there is a poor understanding of how altered neuro-immune communication impacts an individual's outcome to infection. In humans and rodents, SCI leads to maladaptive changes in the spinal-sympathetic reflex (SSR) circuit which is crucial to sympathetic function. The cause of the impaired immune function may be related to harmful neuroinflammation which is detrimental to homeostatic neuronal function, aberrant plasticity, and hyperexcitable circuits. Soluble tumor necrosis factor (sTNF) is a pro-inflammatory cytokine that is elevated in the CNS after SCI and remains elevated for several months after injury. By pharmacologically attenuating sTNF in the CNS after SCI we were able to demonstrate improved immune function. Furthermore, when we investigated the specific cellular population which may be involved in altered neuro-immune communication we reported that excessive TNFR1 activity on excitatory INs promotes immune dysfunction. Furthermore, this observation is NF-kβ dependent in VGluT2 + INs. Our data is the first report of a target within the CNS, TNFR1, that contributes to SCI-induced immune dysfunction after T9-SCI and is a potential avenue for future therapeutics.

Keywords: IKK2; IKKβ; Interneurons; NF-kβ; Spinal cord injury; TNFR1; VGat; VGlut2; Viral immunity.

MeSH terms

  • Animals
  • Female
  • Interneurons* / metabolism
  • Interneurons* / physiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Receptors, Tumor Necrosis Factor, Type I* / metabolism
  • Spinal Cord Injuries* / immunology
  • Spinal Cord Injuries* / metabolism
  • Spinal Cord Injuries* / physiopathology
  • Spinal Cord* / immunology
  • Spinal Cord* / metabolism
  • Tumor Necrosis Factor-alpha* / metabolism
  • Vesicular Glutamate Transport Protein 2* / metabolism

Substances

  • Vesicular Glutamate Transport Protein 2
  • Tumor Necrosis Factor-alpha
  • Receptors, Tumor Necrosis Factor, Type I
  • Slc17a6 protein, mouse
  • Tnfrsf1a protein, mouse
  • XENP 1595