Stimulated Brillouin scattering flow cytometry

Jake R Rosvold; Giulia Zanini; Chenchen Handler; Eric Frank; Jiarui Li; Michele I Vitolo; Stuart S Martin; Giuliano Scarcelli

doi:10.1364/BOE.537602

Stimulated Brillouin scattering flow cytometry

Biomed Opt Express. 2024 Sep 25;15(10):6024-6035. doi: 10.1364/BOE.537602. eCollection 2024 Oct 1.

Authors

Jake R Rosvold¹, Giulia Zanini¹, Chenchen Handler², Eric Frank¹, Jiarui Li¹, Michele I Vitolo^{3

4}, Stuart S Martin^{3

4}, Giuliano Scarcelli¹

Affiliations

¹ Fischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, College Park, MD 20742, USA.
² Department of Mechanical Engineering, A. James Clark School of Engineering, University of Maryland, College Park, MD 20742, USA.
³ Department of Pharmacology, University of Maryland School of Medicine, Baltimore, MD, USA.
⁴ Marlene and Stewart Greenbaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Abstract

We present the use of stimulated Brillouin scattering spectroscopy to achieve rapid measurements of cell biomechanics in a flow cytometer setup. Specifically, our stimulated Brillouin scattering flow cytometry can acquire at a rate of 200 Hz, with a spectral acquisition time of 5 ms, which marks a 10x improvement compared to previous demonstrations of spontaneous Brillouin scattering flow cytometry. We experimentally validate our stimulated Brillouin scattering flow cytometer by measuring cell populations of normal breast epithelial cells and metastatic breast epithelial cancer cells.

Associated data

figshare/10.6084/m9.figshare.26966365