Cerebellar activity in hemi-parkinsonian rats during volitional gait and freezing

Valerie DeAngelo; Arianna Gehan; Siya Paliwal; Katherine Ho; Justin D Hilliard; Chia-Han Chiang; Jonathan Viventi; George C McConnell

doi:10.1093/braincomms/fcae246

Cerebellar activity in hemi-parkinsonian rats during volitional gait and freezing

Brain Commun. 2024 Oct 25;6(5):fcae246. doi: 10.1093/braincomms/fcae246. eCollection 2024.

Authors

Valerie DeAngelo¹, Arianna Gehan¹, Siya Paliwal¹, Katherine Ho¹, Justin D Hilliard², Chia-Han Chiang³, Jonathan Viventi^{3

4

5

6}, George C McConnell¹

Affiliations

¹ Department of Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
² Department of Neurosurgery, University of Florida, Gainesville, FL 32611, USA.
³ Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
⁴ Department of Neurosurgery, Duke School of Medicine, Durham, NC 27710, USA.
⁵ Department of Neurobiology, Duke School of Medicine, Durham, NC 27710, USA.
⁶ Duke Comprehensive Epilepsy Center, Duke School of Medicine, Durham, NC 27710, USA.

Abstract

Parkinson's disease is a neurodegenerative disease characterized by gait dysfunction in the advanced stages of the disease. The unilateral 6-hydroxydopamine toxin-induced model is the most studied animal model of Parkinson's disease, which reproduces gait dysfunction after >68% dopamine loss in the substantia nigra pars compacta. The extent to which the neural activity in hemi-parkinsonian rats correlates to gait dysfunction and dopaminergic cell loss is not clear. In this article, we report the effects of unilateral dopamine depletion on cerebellar vermis activity using micro-electrocorticography during walking and freezing on a runway. Gait and neural activity were measured in 6-hydroxydopamine- and sham-lesioned rats aged between 4 and 5 months at 14, 21 and 28 days after infusion of 6-hydroxydopamine or control vehicle into the medial forebrain bundle (n = 20). Gait deficits in 6-hydroxydopamine rats were different from sham rats at 14 days (P < 0.05). Gait deficits in 6-hydroxydopamine rats improved at 21 and 28 days except for run speed, which decreased at 28 days (P = 0.018). No differences in gait deficits were observed in sham-lesioned rats at any time points. Hemi-parkinsonian rats showed hyperactivity in the cerebellar vermis at 21 days (P < 0.05), but not at 14 and 28 days, and the activity was reduced during freezing epochs in Lobules VIa, VIb and VIc (P < 0.05). These results suggest that dopaminergic cell loss causes pathological cerebellar activity at 21 days post-lesion and suggest that compensatory mechanisms from the intact hemisphere contribute to normalized cerebellar activity at 28 days. The decrease in cerebellar oscillatory activity during freezing may be indicative of neurological changes during freezing of gait in patients with Parkinson's disease making this region a potential location for biomarker detection. Although the unilateral 6-hydroxydopamine model presents gait deficits that parallel clinical presentations of Parkinson's disease, further studies in animal models of bilateral dopamine loss are needed to understand the role of the cerebellar vermis in Parkinson's disease.

Keywords: 6-OHDA; Parkinson’s disease; cerebellar vermis; μECoG.