Effect of enzymatic chemo-mechanical agents on adhesion of composite resin to dentin of permanent teeth: an in vitro study

A S Coelho; L Vilhena; I Amaro; A Melo; A Paula; C M Marto; M M Ferreira; A Ramalho; E Carrilho

doi:10.1007/s40368-024-00949-9

Effect of enzymatic chemo-mechanical agents on adhesion of composite resin to dentin of permanent teeth: an in vitro study

Eur Arch Paediatr Dent. 2024 Nov 1. doi: 10.1007/s40368-024-00949-9. Online ahead of print.

Authors

A S Coelho^{1

2

3

4

5}, L Vilhena⁶, I Amaro^{7

8

9

10

6}, A Melo⁷, A Paula^{7

8

9

10

6}, C M Marto^{8

9

10

6

11}, M M Ferreira^{8

9

10

6

12}, A Ramalho⁶, E Carrilho^{7

8

9

10

6}

Affiliations

¹ Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal. anasofiacoelho@gmail.com.
² Institute for Clinical and Biomedical Research (iCBR), Area of Environment, Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, Coimbra, Portugal. anasofiacoelho@gmail.com.
³ CNC.IBILI Consortium, Faculty of Medicine, University of Coimbra, Coimbra, Portugal. anasofiacoelho@gmail.com.
⁴ Centre for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal. anasofiacoelho@gmail.com.
⁵ Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University Coimbra, 3004-516, Coimbra, Portugal. anasofiacoelho@gmail.com.
⁶ Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University Coimbra, 3004-516, Coimbra, Portugal.
⁷ Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
⁸ Institute for Clinical and Biomedical Research (iCBR), Area of Environment, Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
⁹ CNC.IBILI Consortium, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
¹⁰ Centre for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal.
¹¹ Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
¹² Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.

PMID: 39485663
DOI: 10.1007/s40368-024-00949-9

Abstract

Purpose: To evaluate and compare the effect of two enzymatic chemo-mechanical caries removal agents with conventional caries removal using rotatory instruments on the adhesion of composite resin to dentin of permanent teeth.

Methods: The sample comprised 30 permanent molars with caries lesions extending to the dentin, randomly distributed into three groups (n = 10 each): 1-Caries removal with rotary instruments (control group); 2-Caries removal with Papacárie Duo^® (F&A Laboratório Farmacêutico, São Paulo, Brazil); 3-Caries removal with Brix 3000™ (Brix S.R.L., Carcarañá, Argentina). After caries removal, the specimens were rinsed and dried. Scotchbond Universal™ adhesive (3 M, Saint Paul, Minnesota, USA) was actively applied in self-etch mode and light-cured. Resin composite increments were applied using a silicone mold (3 × 3 × 2 mm) and light-cured. Shear bond strength (MPa), work-to-debonding (J/m²), and shear modulus (kPa) were evaluated. For statistical analysis, the level of significance was set at 5%.

Results: The control group presented significantly higher shear bond strength values (8.50 ± 2.69 MPa) compared to the Brix 3000™ group (5.72 ± 1.55 MPa, p = 0.008). There were no significant differences between Papacárie Duo^® (6.66 ± 0.86 MPa) and the other groups (p > 0.05). Regarding work-to-debonding, the Papacárie Duo^® group had a significantly higher result (2944.41 ± 450.21 J/m²) than the Brix 3000™ group (1189.41 ± 504.13 J/m², p < 0.001) and the control group (967.10 ± 270.01 J/m², p < 0.001). Concerning shear modulus, the control group showed a significantly higher result (558.67 ± 168.96 kPa) than the Brix 3000™ group (339.79 ± 143.78 kPa, p = 0.008) and the Papacárie Duo^® group (223.04 ± 127.30 kPa, p < 0.001).

Conclusion: While the application of Papacárie Duo^® did not negatively affect composite resin adhesion to dentin of permanent teeth, the application of Brix 3000™ reduced adhesive forces, potentially limiting its clinical use. Further investigations are warranted to elucidate the effects of these materials on dentin substrate, particularly through clinical studies.

Keywords: Brix 3000™; Dental bonding; Dental caries; Papacárie Duo®; Shear bond strength.