New evidence of the arsenic uptake and translocation in As-hyperaccumulator fern Pteris cretica using a modified hydroponic system

Yi Huang-Takeshi Kohda; Takaya Hamai; Kengo Horiuchi; Yuki Semoto; Chihiro Inoue

doi:10.1016/j.jhazmat.2023.132855

New evidence of the arsenic uptake and translocation in As-hyperaccumulator fern Pteris cretica using a modified hydroponic system

J Hazard Mater. 2023 Oct 24:463:132855. doi: 10.1016/j.jhazmat.2023.132855. Online ahead of print.

Authors

Yi Huang-Takeshi Kohda¹, Takaya Hamai², Kengo Horiuchi², Yuki Semoto², Chihiro Inoue³

Affiliations

¹ Graduate School of Environmental Studies, Tohoku University, Sendai, Japan. Electronic address: yi.huang.a3@tohoku.ac.jp.
² Technical Research & Consulting Division Metal Environment Management Department, Japan Organization for Metals and Energy Security, Tokyo, Japan.
³ Graduate School of Environmental Studies, Tohoku University, Sendai, Japan. Electronic address: chihiro.inoue.b1@tohoku.ac.jp.

PMID: 39491989
DOI: 10.1016/j.jhazmat.2023.132855

Abstract

Pteris cretica is a native fern in temperate regions known for its ability to hyperaccumulate arsenic (As). However, the uptake and translocation of As in hydroponic conditions have remained poorly understood. In this study, we modified a hydroponic cultivation system, combining 24 plants into one unit, to suppress individual differences among plants and evaluate As uptake and translocation in P. cretica. The 7-day short-term As removal experiment results revealed that the As uptake rate by P. cretica appears to be dependent on the initial As concentration in the hydroponic medium. Furthermore, the rhizomes play a role in regulating As translocation from roots to fronds in P. cretica was observed. In the 27-day long-term continuous flow experiment, approximately 1 mg/d of As was removed by P. cretica with 200 μg/L As applied. Moreover, the long-term translocation factor (TF) between rhizomes and roots and between fronds and rhizomes from the 27-day long-term continuous flow experiment demonstrated the same pattern as the short-term TF from the 7-day short-term As removal experiment, further indicating the role of rhizomes in regulating As translocation from roots to fronds in P. cretica.

Keywords: Arsenic; As-translocation; Hydroponic cultivation; Pteris cretica; Rhizomes.