Protocol to study electrophysiological properties of hPSC-derived 3D cardiac organoids using MEA and sharp electrode techniques

Ravichandra Venkateshappa; Zehra Yildirim; Shane R Zhao; Matthew A Wu; Francesca Vacante; Oscar J Abilez; Joseph C Wu

doi:10.1016/j.xpro.2024.103406

Protocol to study electrophysiological properties of hPSC-derived 3D cardiac organoids using MEA and sharp electrode techniques

STAR Protoc. 2024 Nov 7;5(4):103406. doi: 10.1016/j.xpro.2024.103406. Online ahead of print.

Authors

Ravichandra Venkateshappa¹, Zehra Yildirim¹, Shane R Zhao¹, Matthew A Wu², Francesca Vacante¹, Oscar J Abilez³, Joseph C Wu⁴

Affiliations

¹ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
² Greenstone Biosciences, Palo Alto, CA 94304, USA.
³ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Division of Pediatric Cardiac Surgery, Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA.
⁴ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: joewu@stanford.edu.

Abstract

Continuing advancements in human pluripotent stem cell (hPSC)-derived complex three-dimensional (3D) cardiac tissues require the development of novel technologies or adaptation of existing technologies to understand the physiology of the derived 3D cardiac tissues. In this protocol, we describe the use of multielectrode array (MEA) and sharp electrode electrophysiology techniques to investigate the electrical properties of 3D cardiac organoids. This protocol deciphers the electrical behavior of 3D cardiac organoids at both the single-cell level and tissue level.

Keywords: Cell Biology; Cell culture; Organoids; Stem Cells; Tissue Engineering.